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当前位置: 首页 > 文章 > 冬虫夏草菌丝粗多糖对SP2/0细胞凋亡和p53表达的影响 畜牧兽医学报 2009,40 (1) 117-121
Position: Home > Articles > Effect of Crude Polysaccharides from Mycelium of Cordyceps sinensis on Expression of p53 Gene and Induction of Apoptosis in SP2/0 Cells Chinese Journal of Animal and Veterinary Sciences 2009,40 (1) 117-121

冬虫夏草菌丝粗多糖对SP2/0细胞凋亡和p53表达的影响

作  者：
刘彦威;刘娜;韩博;田向荣;苏敬良
单  位：
河北工程大学农学院;邯郸农业高等专科学校;中国农业大学动物医学院
关键词：
冬虫夏草;粗多糖;SP2/0细胞;p53基因;凋亡
摘  要：
观察冬虫夏草菌丝粗多糖对SP2/0细胞凋亡诱导和p53基因表达的影响。在体外用粗多糖处理SP2/0细胞,MTT法检测其对肿瘤细胞增殖抑制作用,Hochest33342荧光染色观察细胞凋亡的形态学变化,DNA琼脂糖凝胶电泳检测细胞凋亡,流式细胞仪检测细胞的周期,实时荧光定量PCR检测p53基因的表达情况。结果发现,与对照组相比,冬虫夏草粗多糖可抑制SP2/0细胞增殖,诱导出现典型凋亡形态变化,电泳出现特有梯形条带,引起细胞周期阻滞,使p53基因的表达明显改变。这提示冬虫夏草粗多糖对SP2/0细胞生长的抑制,可能与诱导凋亡和p53基因表达的改变有关。
译  名：
Effect of Crude Polysaccharides from Mycelium of Cordyceps sinensis on Expression of p53 Gene and Induction of Apoptosis in SP2/0 Cells
作  者：
LIU Yan-wei1,2,LIU Na1,HAN Bo2,TIAN Xiang-rong2,SU Jing-liang2,ZHAO De-ming2 (1.College of Agriculture,Hebei Engineering University,Handan 056021,China;2.College of Veterinary Medicine,China Agricultural University,Beijing 100193,China)
关键词：
Cordyceps sinensis;crude polysaccharides;SP2/0 cell;p53 gene;apoptosis
摘  要：
The SP2/0 cells were treated with crude polysaccharides from mycelium of Cordyceps sinensis in vitro to explore the effect of crude polysaccharides on induction of apoptosis and expression of p53 gene in SP2/0 cells.MTT assay was used to measure the anti-proliferative effect of crude polysaccharides.Apoptotic morphologic changes of the treated cell were observed by Hochest 33342 staining.Induction of appoptosis was also proved by agarose gele electrophoresis.Cell cycle were analyzed by flow cytometry.Expression of p53 was measured with real-time quantitative PCR.The results were as follows: Crude polysaccharides could inhibit the growth of SP2/0 cell in a dose dependent manner;Typical apoptosis was observed under fluorescence microscope and a fragmented DNA ladder was visualized by agarose gel electrophoresis.In the flow cytometry analysis,arrest of cell cycle was observed in polysaccharides treated cells.Real time quantitative PCR analyses revealed the variation of p53 gene expression in the crude polysaccharides treated Sp2/0 cells.These results suggested that the inhibition of SP2/0 cell by crude polysaccharides may be related to induction of apoptosis and expression of p53 gene in SP2/0 cells.

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