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当前位置: 首页 > 文章 > 嗜线虫致病杆菌Sy1a菌株粗提液杀虫活性的研究 辽宁农业科学 2014 (4) 15-18
Position: Home > Articles > Insecticidal Activity Study of the Crude Extract from Xenorhabdus spp. Strain Sy1a Liaoning Agricultural Sciences 2014 (4) 15-18

嗜线虫致病杆菌Sy1a菌株粗提液杀虫活性的研究

作  者：
庄艳;薛仁风;葛维德;张玲
单  位：
辽宁省农业科学院作物研究所;吉林省农业科学院农业生物技术研究所
关键词：
嗜线虫致病杆菌;粗提液;杀虫活性;总蛋白酶活性
摘  要：
以桃蚜(Myzus persicae),豌豆蚜(Acyrthosiphon pisum Harris)和白粉虱(Trialeurodes?vaporariorum)作为供试昆虫,对Sy1a菌株粗提液的杀虫活性和总蛋白酶活性进行测定。结果表明:桃蚜取食浓度分别为400、600、1 000μg/ml Sy1a菌株粗提液48 h后LC50值为987.7μg/ml,IC50值为5 941.2μg/ml;豌豆蚜取食浓度分别为250、500、1 000μg/ml Sy1a菌株粗提液36 h后,LC50值为227.2μg/ml,IC50值为508.3μg/ml;采用浓度为1 200.0μg/ml的Sy1a菌株粗提液处理后7、14和21 d的温室中白粉虱虫口减退率分别为51.6%、83.7%、89.1%,校正防效分别达到62.0%、88.7%、93.1%。研究结果表明:Sy1a菌株粗提液对桃蚜、豌豆蚜及白粉虱等害虫均有显著的防治效果,具有开发成为环境友好型生物农药的前景。
译  名：
Insecticidal Activity Study of the Crude Extract from Xenorhabdus spp. Strain Sy1a
作  者：
ZHUANG Yan;XUE Ren-feng;GE Wei-de;ZHANG Ling;Crop Research Institute,Liaoning Academy of Agricultural Sciences;Agro-Biotechnology Research Institute,Jilin Academy of Agricultural Sciences;
关键词：
Xenorhabdus spp.;;Crude extract;;Insecticidal activity;;Total protease activity
摘  要：
We tested the insecticidal activity of crude extract from Xenorhabdus spp. strain Sy1 a towards peach aphids( Myzus persicae),pea aphids( Acyrthosiphon pisum Harris) and whiteflies( Trialeurodes vaporariorum). The results indicated the LC50 for mortality and IC50 for protease activitical inhibition caused by treatment of 400,600,1 000 μg /ml crude extract were 987. 7 μg /ml and 5 941. 2 μg /ml towards peach aphids at48 h after feeding; the LC50 for mortality and IC50 for protease activitical inhibition caused by treatment of250,500,1000 μg /ml crude extract were 227. 2 μg /ml and 508. 3 μg /ml towards pea aphids at 36 h after feeding; 1 200 μg /ml crude extract led the population reduction of whiteflies in greenhouse to 51. 6%,83. 7% and 89. 1% at 7,14 and 21 d after spraying and corrected control efficiency were 62. 0%,88. 7%and 93. 1%,respectively. The result all above demonstrated that Xenorhabdus spp. strain Sy1 a has a broad applied perspective in biological control towards insect pests in agriculture as a novel biopesticides.

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