Position: Home > Articles > Isolation and Identification of a High Exopolysaccharide-producing Strain from Biological Soil Crusts on Sand Dunes
FOOD SCIENCE
2011,32
(21)
207-209
沙漠生物结皮高产胞外多糖菌株的筛选与鉴定
作 者：
王正荣;生吉萍;田小磊;武婷婷;刘万振
单 位：
河南科技学院食品学院;中国农业大学食品科学与营养工程学院河南科技学院食品学院;中国农业大学食品科学与营养工程学院
关键词：
沙漠生物结皮;胞外多糖;鉴定;苏云金芽孢杆菌
摘 要：
采用传统的分离纯化方法从沙漠生物结皮中分离到27株产胞外多糖菌株,并通过苯酚-硫酸法从中复筛,筛选到一株产多糖量为7445.80mg/L的菌株XJ-27。根据菌株的形态学特征、生理生化鉴定方法初步鉴定为苏云金芽孢杆菌(Bacillus thuringiensis);通过16S rDNA基因序列分析对该菌株进行了鉴定,PCR扩增得到1452bp的序列,PCR产物序列通过Blast软件在NCBI网站中进行同源性比较,通过MEGA4.0软件绘制系统发育树,结果进一步确认了该菌的地位,XJ-27在细菌系统发育分类学上也归属为苏云金芽孢杆菌。
译 名：
Isolation and Identification of a High Exopolysaccharide-producing Strain from Biological Soil Crusts on Sand Dunes
作 者：
WANG Zheng-rong1,2,SHENG Ji-ping1,TIAN Xiao-lei1,WU Ting-ting1,LIU Wan-zhen1,CHENG Fan-sheng1, JIAO Yu-cui1,SHEN Lin1,(1.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China; 2.College of Food Science,Henan Institute of Science and Technology,Xinxiang 453003,China)
关键词：
biological soil crusts;exopolysaccharides;identification;Bacillus thuringiensis
摘 要：
Twenty-seven stains producing exopolysaccharides were isolated from biological soil crusts in the Gurbantunggut Desert,Xinjiang by the traditional method.A strain with 7445.80 mg/L exopolysaccharide-producing activity was picked out of them by phenol-sulfuric acid method and named as XJ-27.Based on phenotypic and physiological-chemical characterization,XJ-27 was primarily identified as Bacillus thuringiensis and further confirmed through 16S rDNA sequence analysis,resulting in a 1452 bp PCR amplification sequence,homology comparison with NCBI databases using the software Blast and phylogenetic tree construction using the software MEGA 4.0.
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