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Position: Home > Articles > Comparison of Two Determination Methods for Polysaccharide Content in Pteris multifida Poir Journal of Anhui Science and Technology University 2012,26 (5) 38-42

凤尾草多糖两种测定方法的比较

作  者:
周国梁;鲍斌;俞浩;张孝林;刘汉珍;时维静
单  位:
上海中医药大学药学院;安徽科技学院食品药品学院
关键词:
凤尾草;多糖含量;苯酚-硫酸法;蒽酮-硫酸法;紫外分光光度法
摘  要:
目的:为选出一种比较准确、方便测定凤尾草多糖的方法。方法:通过热浸法提取凤尾草中多糖,采用蒽酮-硫酸法、苯酚-硫酸法两种方法进行含量测定比较。结果:蒽酮-硫酸法和苯酚-硫酸法的稳定性实验RSD分别为1.56%和0.68%,精密度实验RSD分别为9.35%和2.28%,回收率平均值分别91.71%和99.23%。结论:苯酚-硫酸法具有稳定性好、精密度高,而且回收率实验也较为理想,是测定凤尾草多糖含量较为理想的方法。
译  名:
Comparison of Two Determination Methods for Polysaccharide Content in Pteris multifida Poir
作  者:
ZHOU Guo-liang1,BAO Bin2,YU Hao1*,ZHANG Xiao-lin1,LIU Han-zhen1,SHI Wei-jing1(1.College of Food and Drug,Anhui Science and Technology University,Fengyang 233100,China; 2.Shanghai Traditional Chinese Medicine University,Shanghai 200032,China)
关键词:
Pteris multifida Poir;Polysaccharide content;Anthrone-sulfuric acid method;Phenol-sulfuric acid method;Uv-spectrophotometry
摘  要:
Objective: In order to select an accurate,convenient method of Pteris multifida Poir polysaccharide determination.Methods: This experiment by hot leaching extracted polysaccharide,the methods of Phenol-sulfuric acid method and Anthrone-sulfuric acid method were compared.Results: The results showed the RSD value of the two methods were 1.56% and 0.68% in stability experiment,9.35% and 2.28% in accuracy experiment,the average recovery was 91.71 % and 99.23% respectively.Conclusion: The experimental design of Phenol-sulfuric acid method is simple,reliable and highly predictive and the result of recovery experiment was more satisfactory.So the better method to determine Pteris multifida polysaccharide content was Phenol-sulfuric acid method.

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