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当前位置: 首页 > 文章 > ‘美人’梅与其近缘种亲缘关系的AFLP研究 园艺学报 2002,29 (6) 588-589
Position: Home > Articles > AFLP Analysis of Genetic Relationships of Prunus mume 'Meiren'and Relatives Acta Horticulturae Sinica 2002,29 (6) 588-589

‘美人’梅与其近缘种亲缘关系的AFLP研究

作  者：
明军;张启翔;毛庆山;晏小兰
单  位：
中国梅花中心;北京林业大学园林学院;湖北农学院园艺系
关键词：
梅;美人梅;亲缘关系;AFLP
摘  要：
以美人梅型的 4个品种以及部分近缘种为试材 ,运用AFLP银染法 ,采用引物组合E ACT/M CAT ,E GA/M CTC ,E ACT/M CCA ,E ACT/M CAC ,扩增得到 4 2 0个位点的 6 30 0条带数据。使用NTSYSpc 2 .1t软件 ,分别采用DICE ,SM和Jaccard匹配系数 ,SAHNClustering进行不加权成对算术平均法(UPGMA)聚类分析 ,得到了‘美人’梅与近缘种的亲缘关系依次为梅、紫叶李、李、山桃、桃花、山杏和杏的结果 ,与形态学、遗传育种学的相关结论相符。
译  名：
AFLP Analysis of Genetic Relationships of Prunus mume 'Meiren'and Relatives
作  者：
Ming Jun 1, Zhang Qixiang 2, Mao Qingshan 3, and Yan Xiaolan 3 ( 1Department of Horticulture, Hubei Agricultural College, Jingzhou 434025, China; 2Beijing Forestry University, Beijing 100083, China; 3The Research Center of China Mei Flower, Wuhan 430000, China)
关键词：
Prunus mume Sieb. et Zucc.; Prunus mume 'Meiren'; Genetic relationships; Amplified Fragment Length Polymorphism
摘  要：
The silver stained AFLP analysis was employed to analyse the DNA fingerprints of four cultivars of Meiren Mei Form, and a wild species, a Pink Double Form and seven relatives. For 420 loci 6 300 bandings was capable of producing sharp fingerprint patterns with the primer combinations of E ACT/M CAT,E GA/M CTC,E ACT/M CCA,E ACT/M CAC. NTSYSpc 2.1 was used to SAHN Clustering of UPGMA and according to DICE coefficient , Jaccard coefficient and Simple Matching coefficient (SM), cluster analysis was done. The relationship among the species was discussed. The result indicated that Prunus mume 'Meiren'was the most close to Prunus mume and Prunus cerasifera 'Pissardii', and distantly related to plum, peach and apricot, conforming to morphological classification and breeding record.

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