作 者：
鄢航;杨秀荣;邓继贤;郭亚芬;兰干球;蒋钦杨;蒋和生
单 位：
广西大学动物科学技术学院;广西科学院/中国东盟(广西)生物技术合作研发中心
摘 要：
利用拼接PCR法从广西眼镜蛇基因组DNA中获得CT成熟肽序列,序列分析显示,广西眼镜蛇与其他地区眼镜蛇的CT基因成熟肽序列具有较高的同源性,同时成功构建了pET30 a(+)-CT原核表达载体;研究结果表明,利用拼接PCR的方法能有效拼接两个外显子序列,广西眼镜蛇CT基因具有较高的保守性,CT基因可在大肠杆菌中表达。
译 名：
Prokaryotic expression of cobrotoxin from Naja naja atra Guangxi
作 者：
YAN Hang1,YANG Xiu-rong1,DENG Ji-xian2,GUO Ya-fen1, LAN Gan-qiu1,JIANG Qin-yang1,JIANG He-sheng1,2(1.College of Animal Science and Technology,Guangxi University,Nanning 530005,China; 2.Guangxi Academy of Sciences/China-ASEAN(Guangxi) Biotechnology Cooperative Center,Nanning 530003,China)
关键词：
Naja naja atra Guangxi;neurotoxin;prokaryotic expression
摘 要：
The objective of current study was to obtain Naja naja atra Guangxi venom neurotoxin — Cobrotoxin(CT) in vitro using splicing PCR.The result showed that CT gene mature peptide sequences was conserved among several species.And prokaryotic expression pET30 a(+)-CT vector was successfully constructed.The current study suggested that using splicing PCR method could effectively spliced two exon sequences;Naja naja atra Guangxi CT gene had a high conservation,and could express in Escherichia coli.
