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Position: Home > Articles > Optimal Extraction and Hydroxyl Radical Scavenging Activity of Water-Soluble Polysaccharides from Pitaya Flowers (Hylocereus undatus (Haw.) Urilt. et. Rose) FOOD SCIENCE 2013,34 (14) 104-107

霸王花水溶性多糖提取工艺及其对羟自由基的清除作用

作  者：
胡位荣;孙茹;李昭露;江月玲;刘顺枝
单  位：
广州大学生命科学学院
关键词：
霸王花;水溶性多糖;浸提;羟自由基
摘  要：
以浸提时间、固液比、浸提温度、提取次数为影响因素,多糖得率为考察指标,在单因素试验的基础上进行正交试验,优化霸王花干制品水提醇沉法提取多糖的工艺条件,并探讨多糖清除羟自由基(.OH)活性。结果表明:霸王花多糖浸提最佳工艺为样品先用乙醇回流处理1h,按固液比1:100(g/mL)加入蒸馏水,90℃水浴浸提4h,浸提1次;浸提液经过醇析后,多糖得率9.59%。对所得多糖的溶解性、吸收光谱等理化性质进行初步观测,霸王花多糖质量浓度为6.4mg/mL时对Fenton体系产生的.OH清除率达50%,15.1mg/mL时可清除90%的.OH。
译  名：
Optimal Extraction and Hydroxyl Radical Scavenging Activity of Water-Soluble Polysaccharides from Pitaya Flowers (Hylocereus undatus (Haw.) Urilt. et. Rose)
作  者：
HU Wei-rong,SUN Ru,LI Zhao-lu,JIANG Yue-ling,LIU Shun-zhi*(School of Life Sciences,Guangzhou University,Guangzhou 510006,China)
关键词：
pitaya flower;water-soluble polysaccharide;water extraction;hydroxyl free radicals
摘  要：
Based on single-factor tests,optimal extraction conditions of hot water extraction and ethanol precipitation of polysaccharides from dried pitaya flowers(Hylocereus undatus(Haw.) Urilt.et.Rose) were explored by orthogonal array design.Factors including extraction duration,temperature,solid-solvent ratio and extraction times were selected to maximize the extraction rate of polysaccharides.The salicylic acid method was used to inspect the hydroxyl radical-scavenging activity of polysaccharides from pitaya flowers.The results showed that the optimal parameters for extracting polysaccharides from pitaya flowers were one cycle of extraction at 90 ℃ for 4 h with distilled water at a solid-to-solvent ratio of 1:100(g/mL) and after ethanol reflux pretreatment for 1 h.Under the optimal extraction conditions,the extraction rate of polysaccharides after ethanol precipitation was 9.59 %.The resulting polysaccharide extract was evaluated for solubility and spectral properties and it was found that its hydroxyl radical-scavenging rate was 50% at 6.4 mg/mL.

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