摘 要：
自山东泰安及烟台地区的小麦全蚀病菌中提取到两种类型颗粒,一类是球形至多边形的病毒颗粒,直径在22～45毫微米之间,多数为27～32毫微米;另一类是棒状颗粒,长度为40～185毫微米×16毫微米,平均108×16毫微米,提取的球形病毒具有核蛋白的典型吸收光谱,最大和最小吸收值的波长分别在260毫微米和240毫微米,A260/240=1.24,A260/280=1.62。以烟台株病毒制备的抗血清,分别与烟台及泰安株病毒在琼脂免疫双扩散中作用均产生沉淀线,表明二者具有共同的血清型。烟台株病毒经苯酚抽提后的核酸样品最大吸收波长是260毫微米,最小是230毫微米,经聚丙烯酰胺—琼脂糖凝胶电泳分离为两条迁移率相近的带,表明此病毒有两个基因片断,在分类上属于需要两个dsRNA片断才能复制的等轴dsRNA真菌病毒。其外壳蛋白经测定含分子量70,000的多肽。从浙江、湖北等11个省(市)的12株全蚀病菌中均提取到了病毒,比较了各菌的菌落颜色,生长速度和病毒浓度。
译 名：
STUDY OF MYCOVIRUS INFECTED GAEUMANNOMYCES(OPHIBOLUS)GRAMINIS
作 者：
Liang Pingyan;Chen Kaiying Instiute of Microbiology,Academia Sinica,Beijing
摘 要：
Two types of virus like particles have been extracted from mycelium of Gaeumannomyces(Ophiobolus)graminis isolated from infected roots of wheat incities Yantai and Taian,Shandong Province.One is spherical to
polyhedral 22-45 nm and predominal 27-32 nm in diameter,the other is
rod-shaped about 16 nm in width and 40-108-185 nm in length with a
broad central canal.The rod-shaped particles were seldom found in ex-
tract.The spherical virus particle preparation exhibited an ultraviolet absor-ption spectrum typical of nucleoprotein with λ maximum at 260 nm and
λ minimum at 240 nm,A260/280=1.62,A260/240=1.24.When the sphe-rical particles of Yantai or Taian isolates were placed in 0.8% agar im-munodiffusion plates and tested against antiserum prepared with virus of Yantai isolate,specific precipitin lines appeared.The result indicated the same serotype between the two isolates.
The dsRNA preparation extracted from virus of Yantai isolate had
a maximum absorbance at 260 nm and minimum absorbance at 230 nm.
When preparations were subjected to electrophoresis in 2.4% polyacryla-mide agarose dis gel,two clear bands of closely similar mobility were obtained,thus showing that the Yantai isolate contained two genome and taxonomically belonged to the possible family i.e.isometric dsRNA mycoviruses requiring two RNA segments for replication.The virus con-tained mainly one capsid polypeptide with a molecular weight of 70,000
dalton.Extracts of 12 isolates collected from nine provinces(Zhejian,Hu-
bei,Neimenggu,Heilongjiang,Jiangsu,Hebei,Shanxi,Shandong,Qing-hai)were examined under the electron microscope,showing that all iso-lates contained spherical virus particles.The colony colourations,growth rates and virus concentration of all these isolates were observed.
