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Position: Home > Articles > STUDIES ON THE SOMATIC EMBRYOGENESIS OF LIRIODENDRON HYBRIDS(L.CHINENSE×L. TULIPIFERA) Scientia Silvae Sinicae 2003,39 (4) 51-55+179

杂交鹅掌楸体细胞胚胎发生研究

作  者:
陈金慧;施季森;诸葛强;黄敏仁
单  位:
南京林业大学林木遗传和基因工程重点实验室
关键词:
杂交鹅掌楸;体细胞胚胎发生;胚性愈伤组织
摘  要:
以杂交鹅掌楸幼胚为外植体 ,进行体细胞胚胎发生研究。比较了亲本基因型、发育阶段、培养条件、生长调节物质和渗透压等对体细胞胚胎发生的影响。结果表明 :在以 1 2MS培养基为基本成分 ,添加 2 0~3 0mg·L- 1 2 ,4 -D的培养基上能够成功诱导胚性愈伤组织。以蔗糖为渗透剂 ,提高渗透压有利于体细胞胚胎发生 ;以MS为基本培养基附加ZT 1 0~ 4 0mg·L- 1 对体细胞胚胎诱导最有效 ;ABA的使用能有效控制畸形胚的产生 ;基因型、幼胚发育阶段及光照条件对体胚发生起着重要作用
译  名:
STUDIES ON THE SOMATIC EMBRYOGENESIS OF LIRIODENDRON HYBRIDS(L.CHINENSE×L. TULIPIFERA)
作  者:
Chen Jinhui\ Shi Jisen\ Zhuge Qiang\ Huang Minren(Key Laboratory of Forestry Genetics and Gene Engineering,Nanjing Forestry University\ Nanjing210037)
关键词:
Liriodendron hybrids, Somatic embryogenesis, Embryonic callus
摘  要:
Somatic embryogenesis was successfully done in Liriodendron hybrids with the immature zygotic embryos as explants. Some factors were tested about their effects on somatic embryogenesis in this research, such as genotype, developmental stage, culture condition, plant growth regulator and osmotic potential. The results showed that the embryogenic callus was successfully induced when the experimental materials were cultured on 1/2 medium supplemented with 2,4-D 2 0~3 0 mg·L -1 . Sucrose could be used as osmoticant to improve osmotic potential of the medium and it was effective for somatic embryogenesis. The induction of somatic embryos was best when the embryonic callus was cultured on MS medium supplemented with 1 0 ~4 0 mg·L -1 ZT. It was also proved that a proper amount of ABA could lessen the production of abnormal embryos. In addition, the genotype , developmental stage of immature embryos and light condition were important to somatic embryogenesis.

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