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当前位置: 首页 > 文章 > 黑龙江腐烂茎线虫群体的分离和鉴定 植物病理学报 2019,49 (6) 756-762
Position: Home > Articles > Isolation and identification of Ditylenchus destructor from Heilongjiang Acta Phytopathologica Sinica 2019,49 (6) 756-762

黑龙江腐烂茎线虫群体的分离和鉴定

作  者：
张淑玲;李惠霞;徐鹏刚;刘永刚;于洪涛;刘向
单  位：
甘肃农业大学植物保护学院/甘肃省农作物病虫害生物防治工程实验室;甘肃省农业科学院植物保护研究所;黑龙江省农业科学院绥化分院
关键词：
腐烂茎线虫;形态特征;rDNA-ITS;基因型
摘  要：
本研究对分离自黑龙江省马铃薯中的1个线虫群体Hp1进行形态观察与测量,采用线虫通用引物对其rDNA-ITS进行扩增与序列分析,利用PAUP软件以ML法构建系统发育树,并测定其致病性。结果显示,该线虫群体有6条侧线,头部略缢缩,口针明显,中食道球呈长纺锤形,食道峡部窄而细长;雌虫阴门稍突起,后阴子宫囊较长;雄虫交合刺略向腹面弯曲,引带短,具交合伞。其形态测量值与腐烂茎线虫(Ditylenchus destructor)基本一致。rDNA-ITS PCR扩增片段916 bp,经BLAST比对,该ITS序列与腐烂茎线虫序列相似度最高。系统发育树显示,该群体没有与腐烂茎线虫A、B基因型群体聚在一起,而是与C、D型群体聚为1个分支,且与C型亲缘关系更近。据此,将群体Hp1鉴定为腐烂茎线虫C基因型,这是首次在黑龙江省发现腐烂线虫为害马铃薯。
译  名：
Isolation and identification of Ditylenchus destructor from Heilongjiang
作  者：
ZHANG Shu-ling;LI Hui-xia;XU Peng-gang;LIU Yong-gang;YU Hong-tao;LIU Xiang;College of Plant Protection of Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province;Institute of Plant Protection,Gansu Academy of Agricultural Sciences;Suihua Branch,Heilongjiang Academy of Agricultural Sciences;
关键词：
Ditylenchus destructor;;morphological characteristics;;rDNA-ITS;;haplotype
摘  要：
The morphological characteristics of a nematode population Hp1 isolated from potato in Heilongjiang were observed and measured in this experiment. The rDNA-ITS of the population was amplified using the universal primers and analyzed and the phylogenetic tree was constructed by ML method of PAUP software. The results showed that Hp1 had 6 lateral lines with slightly constricted head, obvious stylet, oval median bulb and narrow and elongated isthmus of the esophagus. The vulva of female slightly raised with long post-uterine sac. The spi-cule of the male was slightly ventral with short gubernaculum and a bursa. The measurements were largely consistent with Ditylenchus destructor. The amplified product of rDNA-ITS was 916 bp and the sequence shared highest similarity with D. destructor. The population Hp1 was not clustered with type A and B, but clustered into one branch with population of haplotype C and D, and had closer affinity with the type C. Hereby, nematode population Hp1 from the potato was confirmed as haplotype C of D. destructor and this is the first report in Heilongjiang.

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