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当前位置: 首页 > 文章 > 芜菁高频率再生体系的建立及优化 园艺学报 2008,35 (6) 833-840
Position: Home > Articles > Establishment and Optimization of a High-frequency Shoot Regeneration System of Turnip(Brassica rapa L.ssp.rapifera) Acta Horticulturae Sinica 2008,35 (6) 833-840

芜菁高频率再生体系的建立及优化

作  者：
马光;周波;李玉花
单  位：
东北林业大学生命科学学院
关键词：
芜菁;Brassica rapa;TDZ;硝酸银;再生
摘  要：
以3个芜菁品种的带柄子叶和胚轴为外植体,采用苯基噻二唑基脲(thidiazuron,TDZ)配合NAA以及BA配合NAA两种组合研究了适于芜菁离体再生的外植体类型和植物生长调节剂组合。结果表明,带柄子叶为外植体,TDZ7.0mg·L-1+NAA1.0mg·L-1的组合对不定芽分化最为有利。以此离体再生体系为基础,针对AgNO3浓度、苗龄、2,4-D预培养时间3个因素进行优化。结果表明:采用5d苗龄的带柄子叶作为外植体,在含有2,4-D1.0mg·L-1的MS培养基上预培养2d,添加TDZ7.0mg·L-1+NAA1.0mg·L-1+AgNO35.0mg·L-1的MS培养基为分化培养基对芜菁离体再生最为有利,最高分化率可以达到90%左右。分化后得到的不定芽在IBA0.1mg·L-1的MS培养基上生根达100%,移植成活率达95%。
译  名：
Establishment and Optimization of a High-frequency Shoot Regeneration System of Turnip(Brassica rapa L.ssp.rapifera)
作  者：
MA Guang,ZHOU Bo,and LI Yu-hua(College of Life Sciences,Northeast Forestry University,Harbin 150040,China)
关键词：
turnip;Brassica rapa;TDZ;AgNO_3;regeneration
摘  要：
With cotyledonary petiole and hypocotyl explants,the shoot regeneration frequencies of three turnip(Brassica rapa L.ssp.rapifera)cultivars were examined.To achieve a high-frequency regeneration system,the hormone combination of thidiazuron(TDZ)and naphthaleneacetic acid(NAA)was compared with combination of benzyladenine(BA)and NAA on shoot regeneration.The results show that cotyledonary petioles were the best explant and that Murashige and Skoog(MS)medium containing 7.0 mg·L-1 TDZ and 1.0 mg·L-1 NAA was suitable recipe for getting high-frequency shoot regeneration.Based the recipe,the effects of AgNO3 concentration,seedling age,pre-culture time of 2,4-dichlorophenoxyacetic acid(2,4-D)were investigated to optimize the shoot regeneration system.The results suggseted that petiolate cotyledon with seedling age of 5 d cultured in MS medium containing TDZ 7.0 mg·L-1 + NAA 1.0 mg·L-1 + AgNO3 5.0 mg·L-1 followed by pre-culture with 2,4-D 1.0 mg·L-1 for 2 d can be induced with highest-frequency regeneration.The highest shoot formation rate was about 90%.The rooting percentage of shoots was 100% on MS supplemented with 0.1 mg·L-1 indole-3-butyric acid(IBA).The 95% rooted shoots survived in a greenhouse.

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