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当前位置: 首页 > 文章 > 食源性沙门氏菌磁性纳米粒子RT-PCR检测方法的研究 食品科学 2009,30 (22) 293-297
Position: Home > Articles > Detection of Food-borne Salmonella by RT-PCR with Magnetic Nanoparticles FOOD SCIENCE 2009,30 (22) 293-297

食源性沙门氏菌磁性纳米粒子RT-PCR检测方法的研究

作  者：
张弛;楮庆华;孟瑾;杨捷琳;顾鸣;韩奕奕;沈鹤柏;赵渝;郭鲁申;李敏;支援;包建强
单  位：
上海德诺产品检测有限公司;上海出入境检验检疫局;上海海洋大学食品学院;上海师范大学生命与环境科学学院
关键词：
沙门氏菌;磁性纳米粒子;逆转录聚合酶链式反应(RT-PCR)
摘  要：
建立适合食源性沙门氏菌的RT-PCR检测体系。根据沙门氏菌的转录起始因子rpoD设计引物Salmrpod2/5,以rpoD基因的mRNA为检测对象,在样品制备时采用新型的磁性纳米粒子分离mRNA技术,建立快速检测食品中沙门氏菌的逆转录聚合酶链式反应(RT-PCR)的方法。结果表明,Salmrpod2/5引物能有效地将沙门氏菌与其他亲缘关系较近的肠杆菌科细菌区分开来。样品中沙门氏菌添加实验表明,该方法对沙门氏菌的检测下限为10CFU/25ml,检测时间少于18h。该方法具有灵敏、特异、快速的特点,适宜于在食品卫生行业中进行推广及应用。
译  名：
Detection of Food-borne Salmonella by RT-PCR with Magnetic Nanoparticles
作  者：
ZHANG Chi1,CHU Qing-hua2,MENG Jin3,YANG Jie-lin2,GU Ming2,HAN Yi-yi3,SHEN He-bai4,ZHAO Yu4,GUO Lu-shen4,LI Min3,ZHI Yuan4,BAO Jian-qiang1,(1.College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;2.Shanghai Entry-Exit Inspection and Quarantine Bureau,Shanghai 200135,China;3.Shanghai Nod Product Testing Service Co.Ltd.,Shanghai 200436,China;4.College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China)
关键词：
Salmonella;magnetic nanoparticles(MNPs);reverse transcription-polymerase chain reaction(RT-PCR)
摘  要：
The main object of this work was to establish a RT-PCR system suitable for Salmonella detection in foodstuffs.A primer Salmrpod2/5 based on the major sigma factor of Salmonella Methods was designed and mRNA in rpoD gene was analyzed.mRNA from bacteria was isolated by magnetic nanoparticles technique.A sensitive,time saving and specific method of reverse transcription-polymerase chain reaction(RT-PCR) combined with magnetic nanoparticle was developed to detect Salmonella.The results indicated amplification on the site of 260bp in Salmonella-specific gene fragment using the primer Salmrpod2/5.Signals from Salmonella were obtained and no cross-reaction was observed with other members of the Enterobacteriaceae.The detection limit of RT-PCR method was less than 10 CFU in 25 ml samples.The whole analysis took less than 18 h.In summary,this method shows advantages with highly specific,sensitive and quick in detection.It would be very useful for the rapid detection of Salmonella spp.in the food and the health industry.

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