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不同蛋白源饲粮下断奶仔猪小肠中增食欲素受体2的表达特征

作  者:
Shuai Che
单  位:
Animal Nutrition Institute, Sichuan Agricultural University, Ya’an 625014, #N#Chin
关键词:
day;intestine;receptor 2;hcrtr2;weanling;hypocrein
摘  要:
为探讨不同蛋白源饲粮对断奶仔猪小肠中增食欲素受体2(HCRTR2)的表达特征,研究采用实时荧光定量PCR方法检测HCRTR2的mRNA在断奶后分别饲喂动物蛋白替代30%总蛋白饲粮(处理1)及全植物性蛋白饲粮(处理2)0、3、7、14日龄大约克猪空肠中的表达特征。结果表明,不同蛋白源饲粮对HCRTR2基因mRNA的表达均有影响;与断奶0日龄相比,在处理1中,断奶3、7和14日龄时,HCRTR2的表达分别上升了4.30、6.60和4.12倍;处理2中,断奶3、7、14日龄HCRTR2基因mRNA的表达量分别为断奶0日龄时的0.71、1.44和4.50倍。结论:不同蛋白源饲粮对HCRTR2基因mRNA的表达均有影响。
译  名:
Effect of Different Weaning Diets on Expression Pattern of Hypocreins Receptor 2 in Intestine for Weanling Piglets
作  者:
SUN Yun-zi1,CHEN Dai-wen2(1.Institute of Life Science,Guizhou Normal University,Guiyang 550001,China;2.Animal Nutrition Institute,Sichuan Agricultural University,Ya'an 625014,China)
关键词:
Hypocreins Receptor 2;jejunum;expression pattern;weaned piglets
摘  要:
To investigate the impact of different weanling diets on expression pattern of Hypocreins Receptor 2(HCRTR2) in intestine for weanlingpiglets,real-time quantitative reverse transcription PCR(RT-qPCR) was carried out to quantify HCRTR2 expression at age of 0,3,7 and 14 days in weanling piglets fed with the diets 30% milk protein substituted the whole protein(treatment 1) and the vegetable protein(treatment 2).RT-qPCR result showed that HCRTR2 expressed in postnatal intestine of piglets during weanling.In the treatment 1,the expression level increased on day 3,7,14 compared with day 0 with 4.30,6.60 and 4.12 times higher respectively and expression on day 7 being highest.In the treatment 2,the expression level increased 1.44 and 4.50 times on day 7,14 while it decreased 0.71 times on day 3 compared with day 0.In conclusion,the different protein diets affect the expression pattern of Hypocreins Receptor 2 in intestine for weanling piglet.

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