Position: Home > Articles > Studies on fermentation conditions for penicillin G acylase production with a recombinant Bacillus megaterium system
Journal of Central South University of Forestry & Technology
2011,31
(7)
124-129+135
重组巨大芽孢杆菌产青霉素G酰化酶发酵条件研究
作 者：
范超;黎继烈;吴浩;朱晓媛;郝聚喜;丁丽霞;张蕾
单 位：
中南林业科技大学;深圳大学计算机与软件学院
关键词：
重组巨大芽孢杆菌;青霉素G酰化酶;Box-Behnken实验设计;响应面法;发酵条件
摘 要：
对重组巨大芽孢杆菌产胞外青霉素G酰化酶的发酵条件进行优化。先用单因素实验确定最适碳源、氮源以及碳源浓度、氮源浓度、发酵时间和发酵温度的范围。采用Box-Behnken实验设计和响应面分析方法,对重组巨大芽孢杆菌产青霉素G酰化酶的发酵条件进行优化,得出其最佳发酵条件为:葡萄糖32 g/L、胰蛋白胨17 g/L、时间42.5 h、温度34℃,在此条件下青霉素G酰化酶的活力达到10 614±8 U/L,较优化前提高了16.83%。
译 名：
Studies on fermentation conditions for penicillin G acylase production with a recombinant Bacillus megaterium system
作 者：
FAN Chao1,LI Ji-lie1,WU Hao2,ZHU Xiao-yuan1,HAO Ju-xi1,DING Li-xia1,ZHANG Lei1(1.School of Life Science and Technology,Central South University of Forestry and Technology,Changsha 410004,Hunan,China;2.College of Computer and Soft ware,Shenzhen University,Shenzhen 518060,Guangdong,China)
关键词：
recombinant Bacillus megaterium;penicillin G acylase;Box-Behnken experimental design;response surface methodology;fermentation conditions
摘 要：
The fermentation conditions for extracellular penicillin G acylase producted by recombinant Bacillus megaterium were optimized.At first,the optimal carbon,nitrogen and the range of carbon concentration,nitrogen concentration,fermentation temperature and fermentation time were determined by single factor experiments.The experiment used the Box-Behnken experimental design and response surface analytical methodology to optimize the fermentation conditions of production of penicillin G acylase of recombinant Bacillus megaterium.The optimum fermentation conditions were obtained: glucose 32g/L,tryptone 17g/L,time 42.5hours,temperature 34℃.Under these conditions,the activity of penicillin G acylase was 10614±8U/L,being increased by 16.83% compared with 16.83% before optimization.
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