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当前位置: 首页 > 文章 > 高效液相色谱法检测猪肉中安眠酮残留量 食品科学 2010,31 (18) 295-297
Position: Home > Articles > Determination of Methaqualone Residues in Pork by High Performance Liquid Chromatography FOOD SCIENCE 2010,31 (18) 295-297

高效液相色谱法检测猪肉中安眠酮残留量

作  者：
吕飞;朱事康;黄刚;曹维强;吴云普
单  位：
惠州出入境检验检疫局综合技术中心
关键词：
高效液相色谱法;猪肉;安眠酮;固相萃取
摘  要：
建立高效液相色谱法(HPLC)检测猪肉中安眠酮残留量。经过实验确定用二氯甲烷提取,固相萃取(SPE)柱净化,流动相定容后用高效液相色谱仪测定;采用乙腈:乙酸胺溶液(25mmol/L)=40:60(V/V)为流动相,226nm为测定波长,1.0mL/min的流速。在0.020～0.20mg/kg范围内具有良好的线性关系,最低检测限为0.020mg/kg,回收率在70%～110%之间,标准曲线回归方程为Y=6.90×104X+3.71×103,相关系数为0.999。此法操作简便、快速、灵敏、准确,样品处理简便易行,适用于测定猪肉中的安眠酮。
译  名：
Determination of Methaqualone Residues in Pork by High Performance Liquid Chromatography
作  者：
L Fei,ZHU Shi-kang,HUANG Gang,CAO Wei-qiang,WU Yun-pu(Center of Comprehensive Technology,Huizhou Entry-Exit Inspection and Quarantine Bureau,Huizhou 516006,China)
关键词：
high performance liquid chromatography;pork;methaqualone;SPE
摘  要：
A high performance liquid chromatographic method was established to determine the content of methaqualone residues in pork.Sample extraction was carried out using dichloromethane as the extraction solvent,followed by clean-up on a solid-phase extraction column and dillution to a certain volum with the mobile phase composed of acetonitrile and 25 mmol/L ammonium acetate before injection onto HPLC system.The detection wavelength was 226 nm and the mobile phase flow rate was 1.0 mL/min.The calibration curve of the developed method was as follows:Y = 6.90 × 104X +3.71 × 103,with a correlation coefficient of 0.999,which exhibited good linearity over the concentration range from 0.020 to 0.20 mg/kg.The detection limit was 0.020 mg/kg,and the inter-and intra-laboratory average spike recovery rates of methaqualone at three levels were between 70% and 110%.This method has the benefits of simplicity,rapidity,high sensitivity,convenience and good feasibility and most suitable for the determination of methaqualone residues in pork.

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