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Position: Home > Articles > Co-degradation of Methyl Parathion by Pseudomonas stutzeri and Arthrobacter sp. Hunan Agricultural Sciences 2015 (4) 97-101

节杆菌与施氏假单胞菌对甲基对硫磷的共降解研究

作  者:
史延华;任磊;贾阳;闫艳春
单  位:
中国农业科学院研究生院
关键词:
甲基对硫磷;对硝基苯酚;施氏假单胞菌;节杆菌;共降解
摘  要:
为实现对有机磷农药甲基对硫磷的彻底降解,研究利用有机磷农药降解菌施氏假单胞菌YC-YH1与对硝基苯酚降解菌CN2对甲基对硫磷进行共降解试验,同时克隆了参与降解过程的相关基因,并对2株菌株在对甲基对硫磷污染土壤的原位修复中的效果进行研究。结果表明,与YC-YH1单独降解相比,YC-YH1和CN2共降解在处理前32 h无较大差异;但之后,甲基对硫磷和对硝基苯酚的降解速度明显加快,处理48 h后甲基对硫磷即被完全降解,比单独用YC-H1降解时提前了16 h,处理64 h时对硝基苯酚就被完全降解。而土壤修复的研究结果表明,菌株YC-YH1与CN2能够高效降解甲基对硫磷,72 h对土壤中100 mg/kg甲基对硫磷的降解率接近100%,尤以未灭菌土壤中降解效果更好。
译  名:
Co-degradation of Methyl Parathion by Pseudomonas stutzeri and Arthrobacter sp.
作  者:
SHI Yan-hua;LEI Ren;YANG Jia;YAN Yan-chun;Graduate School of Chinese Academy of Agricultural Sciences;
关键词:
methyl Parathion,p-nitropehnol,Pseudomonas stutzeri,Arthrobacter sp.co-degradation
摘  要:
To completely degrade methyl parathion, Pseudomonas stutzeri YC-YH1 and Arthrobacter sp. CN2 were utilized to co-degrade methyl parathion. Cloning of degrading related genes was conducted to investigate the degrading mechanism. The results showed that compared to the degradation of YC-YH1 alone, there were no differences in the first 32 h with the co-degradation of YC-YH1 and CN2.But later, the degradation velocity of parathion-methyl and p-nitrophenol speeded up obviously, only 48 h later, parathion-methyl was degraded completely, and 64 h later, p-nitrophenol was degraded too. In in situ bioremediation assay, strain YC-YH1 and CN2 could efficiently degrade methyl parathion and the degradation rate of 100 mg/kg methyl parathion in soil was almost 100% in 72 hours,especially in non-sterilized soil.

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