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当前位置: 首页 > 文章 > 捕食性真菌Duddingtonia flagrans基因组DNA的提取及基因组survey分析 中国兽医学报 2017,37 (11) 2090-2094
Position: Home > Articles > Genome DNA extraction and genome survey analysis in nematode-trapping fungus-Duddingtonia flagrans Chinese Journal of Veterinary Science 2017,37 (11) 2090-2094

捕食性真菌Duddingtonia flagrans基因组DNA的提取及基因组survey分析

作  者：
张伟;王瑞;杨晓野;祝晓蕊;王新芳;梁萌;杨莲茹;李军燕;罗晓平
单  位：
内蒙古农业大学兽医学院
关键词：
捕食性真菌;Duddingtonia flagrans;DNA提取;基因组测序;GC含量;杂合度
摘  要：
采用透析膜培养法结合DNA柱式提取法进行基因组DNA提取,同时利用第2代高通量测序技术,分析了捕食性真菌Duddingtonia flagrans基因组概况.结果显示:透析膜培养结合DNA柱式提取法所提取的基因组DNA质量及浓度均能达到测序标准;捕食性真菌Duddingtonia flagrans基因组约为37.6 Mb,GC含量为44.95%,基因组杂合度较低.结果表明:透析膜培养法结合拄式法提取其基因组DNA的方法简便可行,为丝状真菌DNA提取,提供了新的思路;基因组survey分析为捕食性真菌Duddingtonia flagrans全基因组精细图谱的绘制奠定了基础.
译  名：
Genome DNA extraction and genome survey analysis in nematode-trapping fungus-Duddingtonia flagrans
关键词：
nematode-trapping fungus%Duddingtonia flagrans%DNA extraction%genome sequencing%GC content%hybridity percentage
摘  要：
Solid culture systems with dialysis membrane were used to culture mycelium.And genome DNA was extracted by spin columns method.Afterwards,the size and complexity of the whole genome of nematode-trapping fungus-Duddingtonia flagrans were determined by the second generation sequencing technology.The quality and concentration of genome DNA was up to a standard for genome sequencing by using the extraction method of solid culture systems with dialysis membrane as well as spin columns.The average genome size of Duddingtoniaflagrans was about 37.6 Mb.And GC content was as high as 44.95% with low hybridity percentage.The DNA extraction method of solid culture systems with dialysis membrane as well as spin columns was simple and feasible,and the genome survey laid a theory foundation for the genome detailed mapping of nematode-trapping fungus-Duddingtonia flagrans.

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