Position: Home > Articles > Studies on Production of Artificial Seeds of Rice Hybrid between Indica and Japonica
Acta Agronomica Sinica
1995
(1)
45-48+131
水稻籼粳杂种人工种子制备的研究
作 者：
邢小黑;尹道川;沈毓渭;高明尉
单 位：
浙江农业大学;江苏省农业科学院
关键词：
水稻籼粳杂种;不定芽;人工种子
摘 要：
从二个水稻籼粳杂种幼穗来源的愈伤组织上,分化出大量不定芽。在2,4-D0.2mg/L、BAP2mg/L的MS培养基上,亚优2号不定芽增殖率可达3.5。无菌条件下,以不定芽作材料制备的人工种子发芽率最高可达60.0％,最低也可达15.0％。使用麦芽糖代替蔗糖,以及用较大的不定芽都可提高人工种子发芽率。
译 名：
Studies on Production of Artificial Seeds of Rice Hybrid between Indica and Japonica
作 者：
Xing Xiaohei Shen Yuwer Gao Mingwei Yin Daochuan"(1. Jiangsu Academy of Agricultural Sciences. Nanjing 210014:2. Zhejiang Agricultural University, Hangzhou 310029)
关键词：
Rice hybrid between Indica and Japonica; Adventitious buds; Artificial seeds
摘 要：
The effects of different factors in artificial seeds of rice hybrid between Indica and Japonica were investigated to establish the basic technology for artificial seeds preparation with improved culture conditions. A large quantity and high frequency of adventitious buds were obtained from calli on MS medium supplemented with 0.2 mg/ L 2,4-D and 2 mg/ L BAP. on which, multiplication coefficient of Yayou 2 hao achieved to 3.5. The germination rate of artificial seeds with encapsulated buds under sterile conditions was from 15.0% to 60.0% on vermiculite and agar bases. To use maltose in place of sucrose and improve quality of adventitious buds could raise germination rate of artificial seeds.
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