当前位置: 首页 > 文章 > 氘代同位素内标液相色谱串联质谱测定肠衣肉类肝脏中5种β受体激动剂类药物残留 中国兽医杂志 2013,49 (7) 80-83
Position: Home > Articles > Determination of 5 kinds of β-agonists in casings,pork and liver with deuterated isotopic internal standard by high performance liquid chromatography coupled with mass spectrometry Chinese Journal of Veterinary Medicine 2013,49 (7) 80-83

氘代同位素内标液相色谱串联质谱测定肠衣肉类肝脏中5种β受体激动剂类药物残留

作  者:
刘谦;颜红
单  位:
保定出入境检验检疫局
关键词:
高效液相色谱-串联质谱;β受体激动剂;痕量残留
摘  要:
建立了肠衣、肉类、肝脏中克伦特罗、莱克多巴胺、氯丙那林、妥布特罗、苯乙醇胺A的液相色谱-串联质谱方法。样品经乙酸铵溶液提取酶解,MCX固相萃取柱净化,流动相溶解后进行检测。5种β受体激动剂标准曲线线性关系良好(r均>0.99);回收率80.0%~98.5%,相对标准偏差为3.4%~8.1%;检出限0.2μg/kg,定量限0.25μg/kg。
译  名:
Determination of 5 kinds of β-agonists in casings,pork and liver with deuterated isotopic internal standard by high performance liquid chromatography coupled with mass spectrometry
作  者:
LIU Qian,YAN Hong(Entry-exit inspection and quarantine bureau of Baoding City,baoding 071051,China)
关键词:
performance liquid chromatography-tandem mass spectrometry;β-agonists;res idue
摘  要:
A performance liquid chromatography-tandem mass spectrometry method was established for the simultaneous determination of clorprenaline,ractopamine,clenbutero,tulobutero,2-(4-(nitrophenyl)butan-2-ylamino)-1-(4-methoxyphenyl) ethanol residues in casings,liver and pork.After enzymolysis,the combined extracts were applied to a solid phase extraction MCX cartridge for cleanup.The average recoveries at three concentrations of 0.25,0.75 and 1.25 μg/kg ranged 80.0%-98.5%.The relative standard deviations of intra-and inter-batch were 3.4%-8.1%.

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