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Position: Home > Articles > Identification and Detection of Pseudomonas syringae pv.tomato in Gansu China Vegetables 2013,1 (6) 68-73

甘肃地区番茄细菌性斑点病菌的鉴定与检测

作  者:
文朝慧;王军平;何苏琴
单  位:
甘肃省农业科学院植物保护研究所;甘肃出入境检验检疫局
关键词:
番茄细菌性斑点病菌;分离;检测
摘  要:
从番茄病果上分离纯化的细菌菌株,经致病性测定、形态特征、培养特性观察及16S rDNA序列测定,鉴定为番茄细菌性斑点病菌Pseudomonas syringae pv.tomato(Okabe)Young,Dye et Wilkie。利用特异性引物对已鉴定菌株及番茄发病组织进行PCR扩增,均扩增出530bp左右的特异性片段,印证了可利用特异性引物MM5F/MM5R对分离菌株或番茄发病组织进行PCR扩增以检测番茄细菌性斑点病菌。
译  名:
Identification and Detection of Pseudomonas syringae pv.tomato in Gansu
作  者:
WEN Zhao-hui 1,WANG Jun-ping 1,HE Su-qin 2*(1 Gansu Entry-exit Inspection and Quarantine Bureau,Lanzhou 730020,Gansu,China;2 Institute of Plant Protection,Gansu Academy of Agricultural Sciences,Lanzhou 730070,Gansu,China)
关键词:
Pseudomonas syringae pv.tomato;Isolation;Detection
摘  要:
Bacteria strains isolated from diseased tomato fruits were identified as Pseudomonas syringae pv.tomato based on pathogenicity,morphological characterizations and 16S ribosomal DNA sequence analysis(99% nucleotide identity).About 530 bp specific fragment of hrpZPst were amplified from these strains and naturally-infected tomato fruits by specific primers MM5F/MM5R.The results confirm that this PCR protocol is suitable for specific detection of P.syringae pv.tomato in field material.

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