Cloning,Bioinfornatics and Tissue Differential Expression Analysis of Pig Transmenbrane and Coiled-coil Domains 2(TMCO2)

PENG Fuzhi;WENG Bo;RAN Maoliang;DONG Lianhua;CHEN Bin;College of Animal Science & Technology,Hunan Agricultural University;Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal;

TMCO2 gene;;cloning;;gene expression;;pig

In this study,the TMCO2 cDNA full length of pig was cloned by RACE and analyzed by bioinformatics,the TMCO2 gene expression levels were detected in Shaziling pig D30 period of eight different tissues( testis,muscle,lung,heart,kidney,liver,spleen,fat) and eight different developmental stages( E90,D1,D30,D60,D90,D120,D150,D180) of testicular tissue by real-time fluorescence quantitative PCR. The results showed that the length of TMCO2 cDNA and the ORF region were 740 bp and 546 bp,respectively,which encoded 181 amino acids. qRT- PCR results showed that the D30 period's highest expression level of TMCO2 mRNA was found in testis,followed by spleen tissues,while the expression level of the other tissues were extremely low,the expression levels between testis and other tissues differed significantly( P < 0.05). In the testis tissue,the expression level of TMCO2 mRNA was the highest in D180 periods,while declined in D1 and D150,the differences between D180 and the other periods were significant( P<0.01). The growing and development stages of E90,D1,D30 and D60 had no significant differences( P > 0. 05). The full length of TMCO2 cDNA was cloned and the expression levels of TMCO2 gene in different tissues and different developmental stages of testes were detected in this study,which provided the foundation for further understanding functions of the TMCO2 gene in pig.