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Position: Home > Articles > Callus Induction in Schisandra sphenanthera Rehd. Et Wils. Nonwood Forest Research 2007,25 (4) 54-56

华中五味子的愈伤组织诱导

作  者:
吴玲娜;谢碧霞;邓白罗;刘伟;李永欣;李俊彬
单  位:
中南林业科技大学
关键词:
华中五味子;组织培养;嫩叶;愈伤组织;外植体
摘  要:
为建立完善的华中五味子组培快繁体系,以嫩叶为外植体,探讨了不同因素对华中五味子组织培养中愈伤组织诱导的影响。结果表明:75%酒精浸泡10 s和0.1%升汞消毒4 min组合的灭菌效果较好;最适培养基为MS培养基,附加2,4-D 0.5 mg/L、NAA 0.1 mg/L、6-BA 1.0 mg/L时,诱导愈伤组织的效果较好,诱导率高达96.7%,且愈伤组织呈淡绿色,质地致密,生长旺盛。
译  名:
Callus Induction in Schisandra sphenanthera Rehd. Et Wils.
作  者:
WU Ling-na,XIE Bi-xia,DENG Bai-luo,LIU Wei,LI Yong-xin, LI Jun-bin(Central South University of Forestry & Technology,Changsha 410004,Hunan,China)
关键词:
Schisandra sphenanthera Rehd.Et Wils.;tissue culture;tender leaf;callus;explants
摘  要:
In order to improve techniques of tissue culture in Schisandra sphenanthera Rehd.Et Wils.,effects of different factors on callus induction were researched,taken tender leaves as explants.The results showed that it was the optimum disinfection method to immerse materials in 75% ethanol for 10 seconds and 0.1% mercuric chloride for 4 minutes;MS was the optimum media,supplemented with 2,4-D 0.5 mg/L,NAA 0.1 mg/L and 6-BA 1.0 mg/L,in which the induction rate was up to 96.7%,and callus were light green,dense texture,vigorous.

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