Position: Home > Articles > Rapid Determination of Crude Protein in Vigna minima Seeds by Microwave Programmed Digestion-Conductivity Micro-titration
FOOD SCIENCE
2012,33
(12)
196-199
微波程序消解-电导率微量滴定法快速测定贼小豆中的粗蛋白
作 者：
高向阳;王惠荣;任婷婷
单 位：
河南农业大学食品科学技术学院
关键词：
微波程序消解;电导率;微量滴定法;粗蛋白质;贼小豆
摘 要：
为建立一种快速测定食品中粗蛋白质的新型分析方法,选择郑州地区野生贼小豆为样品,用硫酸-过氧化氢混合溶液为消解剂,利用微波压力程序消解技术,将样品中的粗蛋白处理为NH4+,用中性甲醛将NH4+转化为H+后,用NaOH标准溶液以电导微量滴定法测定。结果表明:贼小豆样品中粗蛋白质含量均值为23.41%,平行测定的相对标准偏差为1.5%(n=5)。与经典标准方法对照测定,经过F值检验和t值检验表明无显著性差异(置信度为95%)。该法具有简便、灵敏、快速、准确、直观、无需对样品消解液进行蒸馏和使用指示剂等显著优点,结果令人满意。
译 名：
Rapid Determination of Crude Protein in Vigna minima Seeds by Microwave Programmed Digestion-Conductivity Micro-titration
作 者：
GAO Xiang-yang,WANG Hui-rong,REN Ting-ting(College of Food Science and Technology,Henan Agricultural University,Zhengzhou 450002,China)
关键词：
microwave program digestion;conductivity;micro-titration method;crude protein;Vigna minima
摘 要：
A new analytical method to rapidly determine protein content in Vigna minima seeds was established in the present study.Samples were digested with a sulfuric acid-hydrogen peroxide solution by microwave programmed digestion to convert crude protein to NH4+.The NH4+ generated in the solution were further converted to H+ ions for conductivity micro-titration with standard NaOH solution.The results showed that the average content of protein in wild Vigna minima seeds from Zhengzhou was determined to be 23.41% with relative standard deviation(RSD) of 1.5%(n = 5).Compared with the classic standard determination method,the conductivity micro-titration did not exhibit significant difference as analyzed by F-test and t-test at 95% confidence level.This method has the advantages of convenient operation,high sensitivity,quick determination,accurate results and no requirements for indicators.
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