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当前位置: 首页 > 文章 > 基于GISH的甘蔗与斑茅F_1染色体遗传与核型分析 植物遗传资源学报 2014,15 (2) 394-398
Position: Home > Articles > Chromosome Genetic and Karyotype Analysis of F_1 Hybrids between Saccharum officinarum and Erianthus arundinaceus Based on GISH Journal of Plant Genetic Resources 2014,15 (2) 394-398

基于GISH的甘蔗与斑茅F_1染色体遗传与核型分析

作  者：
黄永吉;吴嘉云;刘少谋;邓祖湖;符成;林彦铨;李奇伟;黄忠兴;陈如凯
单  位：
广州甘蔗糖业研究所;福建农林大学/农业部福建甘蔗生物学与遗传育种重点实验室
关键词：
甘蔗;斑茅;染色体;基因组原位杂交;核型
摘  要：
斑茅在甘蔗育种的利用是现代甘蔗育种种质创新的热点,育种者期望把斑茅中优异的特性通过杂交渗透到甘蔗中。甘蔗与斑茅的F1是斑茅利用研究的难点也是基础。本研究利用基因组原位杂交技术(GISH)分析甘蔗与斑茅的F1染色体构成和核型,探讨甘蔗与斑茅F1染色体的遗传行为。GISH结果表明,甘蔗与斑茅杂交F1的染色体众数68~69条,其中40条来自甘蔗热带种Badila,28~29条来自海南斑茅,未发现有染色体的交换或易位现象。参试材料大部分染色体都属于中部着丝点(m)的染色体,少数为近中部着丝点(sm),YCE95-41核型属2B型,其余的核型都为1B型。甘蔗与斑茅的染色体按n+n的方式传递给F1,本研究结果为斑茅种质在甘蔗育种中的利用及其杂交后代染色体细胞遗传研究提供参考依据。
译  名：
Chromosome Genetic and Karyotype Analysis of F_1 Hybrids between Saccharum officinarum and Erianthus arundinaceus Based on GISH
作  者：
HUANG Yong-ji;WU Jia-yun;LIU Shao-mou;DENG Zu-hu;FU Cheng;LIN Yan-quan;LI Qi-wei;HUANG Zhong-xing;CHEN Ru-kai;Key Lab of Sugarcane Biology and Genetic Breeding,Ministry of Agriculture / Fujian Agriculture and Forestry University;Guangzhou Research Institute for Sugarcane Industry;
关键词：
Saccharum officinarum;;Erianthus arundinaceus;;chromosome;;genomic in situ hybridization;;karyotype
摘  要：
Utilization of Erianthus arundinaceus is a hot topic in modern sugarcane germplasm innovation program. Sugarcane breeders expect to permeate excellent characteristics of Erianthus arundinaceus into Saccharum through intergeneric cross. The F1hybrids perform a base and importance role in studying and utilization of Erianthus arundinaceus. In this study,we characterized the karyotype and chromosome behavior of F1hybrids between Saccharum officinarum and E. arundinaceus using genomic in situ hybridization( GISH). The results showed that there were 68 to 69 chromosomes in the F1hybrids,of which 40 were from S. officinarum,and 28 to 29 were from E. arundinaceus. Chromosome translocation and exchange were not observed in the F1hybrids. Most of chromosomes were classified as median centromere type( m) and few of chromosomes were submetacentromere type( sm) in 5 F1clones. The karyotype of YCE95-41 falled into 2B,the others falled into 1B. GISH result revealed that the F1between S. officinarum and E. arundinaceus resulted from n + n chromosome transmission. Our study provided fundamental information for utilization of E. arundinaceus germplasm into sugarcane breeding program.

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