当前位置: 首页 > 文章 > 厚壳贻贝血细胞颗粒的蛋白质组学分析 南方水产科学 2012,08 (2) 9-16
Position: Home > Articles > Proteomic analysis of granule of hemocytes from Mytilus coruscus South China Fisheries Science 2012,08 (2) 9-16

厚壳贻贝血细胞颗粒的蛋白质组学分析

作  者:
王信超;孙敬敬;范美华;武梅;石戈;王日昕;廖智
单  位:
海洋生物资源及分子工程实验室;浙江海洋学院海洋科学学院
关键词:
厚壳贻贝;血细胞颗粒;LTQ-Orbitrap;质谱;Shotgun
摘  要:
贝类的血细胞在宿主的免疫防御机制中发挥着重要的作用。文章利用线性离子阱-四级杆质谱对厚壳贻贝(Mytilus coruscus)血细胞的颗粒蛋白质组分进行了Shotgun分析,结合Mascot数据库搜索,共鉴定了125种高丰度蛋白,其中包括细胞骨架蛋白、细胞粘附蛋白、精氨酸激酶和代谢相关酶类等。此结果为深入了解厚壳贻贝血细胞颗粒的蛋白组成、贻贝免疫分子标记的筛选及其免疫机制奠定了基础。
译  名:
Proteomic analysis of granule of hemocytes from Mytilus coruscus
作  者:
WANG Xinchao,SUN Jingjing,FAN Meihua,WU Mei,SHI Ge,WANG Rixin,LIAO Zhi(Lab.of Marine Biological Source and Molecular Engineering,College of Marine Sciences, Zhejiang Ocean University,Zhoushan 316004,China)
关键词:
Mytilus coruscus;hemocytes granule;LTQ-Orbitrap;mass spectrometry;Shotgun
摘  要:
The blood cells of mussel play an important role in the host′s immune defense mechanism.We explore the protein composition in the granule of hemocytes from Mytilus coruscus by using linear-ion-trap-quadrupole mass spectrometry(LTQ-Orbitrap) combined with Shotgun strategy.A total of 125 high-abundance proteins are identified by Mascot searching methods,including cytoskeletal proteins,cell adhesion proteins,arginine kinases and metabolism-related enzymes,etc..The results lay a foundation for further studies on the composition of mussel protein particles,screening of its immune molecular markers as well as its immune mechanism.

相似文章

计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊