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当前位置: 首页 > 文章 > 猪源杀鲑气单胞菌分离鉴定及毒力因子检测 中国兽医杂志 2019 (5) 74-76+79+125
Position: Home > Articles > Characterization of antibiotic resistance and detection of virulence genes in Aeromonas salmonicida isolated from pig Chinese Journal of Veterinary Medicine 2019 (5) 74-76+79+125

猪源杀鲑气单胞菌分离鉴定及毒力因子检测

作  者：
邓同炜;蒋增海;霍元楠;宋浩;何启盖
单  位：
河南牧业经济学院动物医学院
关键词：
猪;杀鲑气单胞菌;分离、鉴定;毒力因子
摘  要：
为了确定河南省某猪场导致保育仔猪死亡的病原菌,从病死猪的肺脏、肝脏和脾脏分离得到一株优势菌。结果显示,经16S rDNA测序确定该优势菌为杀鲑气单胞菌;动物致病试验显示,分离菌株对小鼠具有致病性;药敏试验显示,该分离菌对磺胺异噁唑、四环素、土霉素、阿莫西林/克拉维酸、卡那霉素、氨苄西林等耐药;毒力基因PCR检测显示,act、ast、fla、lipase毒力基因为阳性,alt、elastase为阴性。本研究首次从发病猪体内分离到杀鲑气单胞菌,并为该条件致病菌的诊疗提供参考依据。
译  名：
Characterization of antibiotic resistance and detection of virulence genes in Aeromonas salmonicida isolated from pig
作  者：
DENG Tong-wei;JIANG Zeng-hai;HUO Yuan-nan;SONG Hao;HE Qi-gai;Couege of Veterinary Medicine,Henan University of Animail Husbandry and Economy;Henan Engineering and Technology Research Conter of Veterinary Biotechnology;State Key Laboratory of Agricultwval Microbiology,Huazhong Agrialltural University;
关键词：
pig;;Aeromonas salmonicida;;isolation and identification;;virulence factor
摘  要：
To investigate the pathogenic bacteria which caused the piglets dead at a farm located in Henan province, a strain of the dominant bacterium was isolated from lungs, heart and liver of a dead piglet. The isolate was identified as Aeromonas salmonicida by 16 S rDNA PCR amplification and sequencing. Animal pathogenicity test showed that the isolate had pathogenicity to mice. The result of Antimicrobial susceptibility test by the disc diffusion method indicated that the isolate was resistant to sulfamethoxazole, tetracycline, oxytetracycline, amoxicillin-clavulantic acid, kanamycin, ampicillin and so on.This study could provide the reference basis for clinical treatment of the conditional pathogen.

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