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当前位置: 首页 > 文章 > 甘南藏区牦牛隐性乳房炎病原菌分离及鉴定 中国草食动物科学 2018 (4)
Position: Home > Articles > 甘南藏区牦牛隐性乳房炎病原菌分离及鉴定 China Herbivore Science 2018 (4)

甘南藏区牦牛隐性乳房炎病原菌分离及鉴定

作  者：
王斐;王彩莲;郎侠;宋淑珍;杨峰;吴建平
关键词：
甘南藏区;牦牛;隐性乳房炎;病原菌
摘  要：
为调查甘肃省甘南州玛曲县、夏河县牦牛隐性乳房炎流行情况,探明引起牦牛隐性乳房炎的主要病原菌,利用兰州乳房炎诊断试剂(LMT)筛选牦牛隐性乳房炎,检出率10.25%;采集非阴性乳样,对其中的病原菌分离纯化,提取细菌DNA并测序后,通过NCBI中BLAST比对,共鉴定出236株细菌,其中包含102株金黄色葡萄球菌(43.22%)、18株艾格尼丝葡萄球菌(7.6%)、13株沃氏葡萄球菌(5.50%)和29株大肠埃希氏菌(12.29%);艾格尼丝葡萄球菌为国内首次自牦牛乳中分离得到.结果表明,葡萄球菌属和埃希氏菌属可能是引起甘南藏区牦牛隐性乳房炎的主要病原菌.
作  者：
Wang Fei;Wang Cailian;Lang Xia;Song Shuzhen;Yang Feng;Wu Jianping;Animal Husbandry-Pasture and Green Agriculture Institute,Gansu Academy of Agricultural Sciences;Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS;Gansu Academy of Agricultural Sciences;
单  位：
Wang Fei%Wang Cailian%Lang Xia%Song Shuzhen%Yang Feng%Wu Jianping%Animal Husbandry-Pasture and Green Agriculture Institute,Gansu Academy of Agricultural Sciences%Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS%Gansu Academy of Agricultural Sciences
关键词：
Tibetan-inhabited regions in Gansu;;yak;;subclinical mastitis;;pathogen
摘  要：
The aim of this study was to investigate the prevalence of yak subclinical mastitis in Maqu and Xiahe in Gansu province,as well as to study the main pathogens causing yak subclinical mastitis. Lanzhou Mastitis Test was used to detect the subclinical mastitis and the positive rate was 10.25%. All the non-negative samples were collected to isolate and identify the pathogens through16 S ribosomal DNA identification and NCBI BLAST. Overall,236 strains of bacteria were identified including Staphylococcus.aureus(102,43.22%),S. agnetis(18,7.6%),S. warneri(13,5.50%) and Escherichia. coli(29,12.29%). Results showed that Staphylococcus sp. and Escherichia sp. should be the main cause of yak subclinical mastitis in Tibetan-inhabited regions in Gansu.

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