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Position: Home > Articles > Cloning and Sequence Analysis of CYP305B1V1 Gene of Bom byx mandarina M. Science of Sericulture 2006,32 (2) 161-165

野桑蚕CYP305B1V1基因的克隆与序列分析

作  者:
陈玉华;卫正国;李兵;王东;赵华强;沈卫德
单  位:
苏州大学蚕桑研究所苏州大学生命科学学院
关键词:
野桑蚕;CYP305B1V1基因;克隆;序列分析
摘  要:
对野桑蚕CYP305B1V1基因进行克隆和序列分析的结果显示,野桑蚕CYP305B1V1基因的ORF为1 464nt,编码487 aa;同源性分析表明,在DNA水平上野桑蚕CYP305B1V1基因与家蚕CYP305B1基因的同源性达99%,与推导的氨基酸序列完全一致。通过和NCB I中家蚕基因组数据比对和拼接,预测野桑蚕CYP305B1V1基因结构中至少含有6个内含子,且内含子与外显子之间的连接符合GT-AT法则。
译  名:
Cloning and Sequence Analysis of CYP305B1V1 Gene of Bom byx mandarina M.
作  者:
CHEN Yu-Hua WEI Zheng-Guo LI Bing WANG Dong ZHAO Hua-Qiang SHEN Wei-De~*(Institute of Sericulture,School of Life Sciences,Soochow University,Suzhou Jiangsu 215123,China)
关键词:
Bombyx mandarina;CYP305B1V1 gene;Cloning;Sequence analysis
摘  要:
The CYP305B1V1 gene from Bombyx mandarina M.was cloned and sequenced.The results showed that the length of ORF of CYP305B1V1 is(1 464) nt,which encodes 487 aa.Homologous analysis showed that the DNA homology of CYP305B1V1 of silkworm and wild silkworm reaches 99%,which is consistent with deduced amino acid sequences.We presume that CYP305B1V1 of wild silkworm contains at least 6 introns and they all obey the GT-AG rule by searching and aligning the silkworm genome database on NCBI.

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