Position: Home > Articles > Identification and Biological Characteristics of Bacterial Strain CICC 10822 Isolated from Aldos Life Nutrition Liquid
Journal of Anhui Agricultural Sciences
2016
(7)
107-110
特色饮料“生命营养液”内源菌株CICC 10822的鉴定及生物学特性
作 者：
曹艳花;亚森·克力木;姚粟;刘洋;翟磊;赵婷;程池
单 位：
中国食品发酵工业研究院中国工业微生物菌种保藏管理中心;新疆埃杜升生物开发有限公司
关键词：
生命营养液;CICC 10822;鉴定;酸鱼乳杆菌;生物学特性
摘 要：
[目的]鉴定特色饮料"生命营养液"内源菌株CICC 10822并了解其生物学特性。[方法]采用传统微生物学分离培养方法对30℃条件下分离得到的新疆埃杜升"生命营养液"细菌CICC 10822进行多相分类鉴定。[结果]菌株CICC 10822接触酶反应阴性,硝酸盐还原阴性,淀粉水解阴性,精氨酸双水解酶阴性;能利用葡萄糖、果糖、核糖和半乳糖,不能利用麦芽糖、木糖、甘油、乳糖、甘露糖、山梨醇、纤维二糖、鼠李糖、阿拉伯糖,不液化明胶。利用基于形态学观察、生理生化特征分析及16S r DNA和phe S基因序列系统发育分析的微生物多相分类鉴定技术,将菌株CICC 10822鉴定为酸鱼乳杆菌(Lactobacillus acidipiscis)。[结论]试验结果为今后开展针对该菌的生物学功能研究奠定了基础。
译 名：
Identification and Biological Characteristics of Bacterial Strain CICC 10822 Isolated from Aldos Life Nutrition Liquid
作 者：
CAO Yan-hua;Yasin kirim;YAO Su;CHENG Chi;China Industrial Microbial Strains Preservation and Management Center,China Food Fermentation Industry Research Institute;Xinjiang ALDOS Biological Development Co.Ltd.;
关键词：
Life nutrition liquid;;CICC 10822;;Identification;;Lactobacillus acidipiscis;;Biological characteristics
摘 要：
[Objective] The aim was to identify bacterial strain CICC 10822 isolated from Aldos life nutrition liquid and under the biological characteristics. [Method] By using the traditional microbiology separation and culture method,bacterial strain CICC 10822 isolated from Xinjiang Aldos life nutrition liquid in 30 ℃ was classified and identified. [Result] Strain CICC 10822 contact enzyme reaction was negative,nitrate reduction was negative,starch hydrolysis was negative,and arginine was negative. It could utilize glucose,fructose,ribose and galactose,and could not use maltose,xylose,lactose,glycerol,mannitol,sorbitol,cellobiose,rhamnose,arabinose,liquefied gelatin. By using microbial taxonomy and identification technology based on morphological observation,physiological and biochemical characteristics analysis,phylogenetic analysis of 16 S r DNA and phe S gene sequences,strain CICC 10822 was identified as Lactobacillus acidipiscis. [Conclusion] The results lay a foundation for study on the bacterial biological functions in the future.
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