当前位置: 首页 > 文章 > 北冬虫夏草固体发酵产胞外多糖条件的研究 安徽农业科学 2013 (18) 7792-7795
Position: Home > Articles > Study on Exopolysaccharide Production with Solid-fermentation Conditions by Cordyceps militaris Journal of Anhui Agricultural Sciences 2013 (18) 7792-7795

北冬虫夏草固体发酵产胞外多糖条件的研究

作  者:
谭彦琦;郭烨;龚秋红;兰时乐
单  位:
湖南农业大学生物科学技术学院
关键词:
北冬虫夏草;固体发酵;胞外多糖;正交试验
摘  要:
[目的]研究北冬虫夏草固体发酵产胞外多糖的条件。[方法]采用固体发酵法培养北冬虫夏草,研究碳源和氮源种类、酵母粉含量、KH2PO4含量、初始pH值、接种量、种龄及发酵时间对北冬虫夏草发酵产胞外多糖的影响。[结果]单因素试验和正交试验表明,最佳的发酵条件为:大米∶米糠=90∶10,酵母粉1.0%、KH2PO40.25%、初始pH 6.0、种龄4 d、接种量8%(V/W)、温度28℃、固水比1∶1(W/V)、发酵时间6 d。在此发酵条件下,北冬虫夏草胞外多糖产量达38.625 mg/g.干基。[结论]该研究为开发新型动物保健饲料提供了理论支持。
译  名:
Study on Exopolysaccharide Production with Solid-fermentation Conditions by Cordyceps militaris
作  者:
TAN Yan-qi;College of Bioscience and Biotechnology,Hunan Agricultural University;
关键词:
Cordyceps militaris;;Solid fermentation;;Exopolysaccharide;;Orthogonal test
摘  要:
[Objective] The aim was to study the solid-fermentation conditions of exopolysaccharide production by Cordyceps militaris.[Method] C.militaris was cultured by solid-fermentation method,and the effects of carbon sources,nitrogen sources,yeast powder content,KH 2 PO 4 content,initial pH value,amount of inoculum,seed age and fermentation time on the exopolysaccharide production of C.militaris were studied.[Result] The single factor tests and orthogonal test showed that,the best fermentation conditions were as follows∶ rice∶ rice bran = 90∶ 10,yeast powder 1.0%,KH 2 PO 4 0.25%,initial pH 6.0,seed age 4 days,amount of inoculum 8%(V / W),temperature 28 ℃,solid-water ratio 1∶ 1(W / V),fermentation time 6 days.Under these conditions,the yield of exopolysaccharides reached 38.625 mg / g.dry substrate.[Conclusion] The study provided theoretical support for the development of new health feed of animal.

相似文章

计量
文章访问数: 14
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊