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当前位置: 首页 > 文章 > 退化与正常山核桃林土壤细菌多样性比较研究 浙江林业科技 2017,37 (5) 42-47
Position: Home > Articles > Comparison on Soil Bacteria Diversity between Degrated and Healthy Carya cathayensis Forests Journal of Zhejiang Forestry Science and Technology 2017,37 (5) 42-47

退化与正常山核桃林土壤细菌多样性比较研究

作  者：
李皓;董建华;董建明;袁紫倩;胡俊靖;赵伟明
关键词：
山核桃;退化;土壤细菌群落;16SrDNA测序;多样性
摘  要：
2014年10月,在浙江省临安市选择4个退化山核桃Carya cathayensis林样地和2个正常山核桃林样地,采土样后用16Sr DNA测序技术分析两种林地土壤细菌多样性。结果表明,6个样地土壤细菌共有36门75纲96目224科924属。在门的水平上,退化和正常林地细菌主要类群基本一致,主要类群有变形菌门Proteobacteria,酸杆菌门Acidobacteria,拟杆菌门Bacteroidetes,绿弯菌门Chloroflexi,厚壁菌门Firmicutes,疣微菌门Verrucomicrobia,放线菌门Actinobacteria和浮霉菌门Planctomycetes,但不同林地中各类群相对丰度明显不同;在属的水平上,退化和正常林地差别很大。Alpha多样性分析显示,6个样地Shannon指数,ACE指数,Chao1指数的排序各不相同,分析说明山核桃林退化程度与土壤细菌数量和多样性无关。相关分析结果表明,ACE指数与交换性H+显著相关,其余土壤物理化学性质与细菌多样性指数均无显著相关性。
译  名：
Comparison on Soil Bacteria Diversity between Degrated and Healthy Carya cathayensis Forests
作  者：
LI Hao;DONG Jian-hua;DONG Jian-ming;YUAN Zi-qian;HU Jun-jing;ZHAO Wei-ming;Hangzhou Forestry Academy of Zhejiang;Lin'an Forestry Bureau of Zhejiang;
关键词：
Carya cathayensis;;degraded;;soil bacteria community;;16SrDNA sequencing;;diversity
摘  要：
Four sample plots in degraded Carya cathayensis stand and 2 in normal ones were established in October 2014 in Lin'an district, Zhejiang province. Soil samples were sequenced by 16 SrDNA for comparison on bacteria community diversity of different sample plots. The results indicated that there were 924 genera of 224 families and 36 phylums, 75 classes, 96 orders in sampled plots. The main phylums were the same in the tested plots, included Proteobacteria, Acidobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Verrucomicrobia, Actinobacteria and Planctomycetesbut with different relative abundance. The distribution of main genera had great difference between degraded and normal plots. The Shannon index, the ACE index and Chao1 index showed that it had no relationship between degradation of sampled stands with bacteria richness and diversity. Correlation analysis demonstrated that ACE index had evident relation with H+, the other physiochemical properties of the tested had no significant correlation with bacteria diversity index.

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