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当前位置: 首页 > 文章 > 漾濞大泡与云新高原核桃花粉活力及柱头可授性比较 西南农业学报 2017 (6) 1434-1438
Position: Home > Articles > Study on Pollen Viability and Stigma Receptivity of Two Walnut Cultivars Yangbidapao and Yunxingaoyuan Southwest China Journal of Agricultural Sciences 2017 (6) 1434-1438

漾濞大泡与云新高原核桃花粉活力及柱头可授性比较

作  者：
胡青;肖良俊;吴涛;宁德鲁;陈少瑜
单  位：
云南省木本油料工程技术研究中心;云南省林业科学院
关键词：
深纹核桃;漾濞大泡;云新高原;花粉活力;柱头可授性
摘  要：
【目的】明确漾濞大泡和云新高原2个核桃品种的花粉活力和柱头可授性。【方法】用氯化三苯基四氮唑(TTC)染色法测定花粉在3种贮藏条件下(室温、冰箱冷藏和冷冻)的活力变化;采用联苯胺-过氧化氢法、过氧化物酶-酯酶试纸法、苏丹红染色法3种方法测定不同时期雌花柱头可授性。【结果】在相同保存温度下,云新高原核桃的花粉活力高于漾濞大泡。冰箱冷藏条件有利于花粉保存,保存至第7天时漾濞大泡和云新高原的花粉活力分别从第1天的88.7%和95.5%下降至10.0%和18.3%。氧化物酶-酯酶试纸法检测柱头可授性操作简单、效果较好。【结论】漾濞大泡和云新高原核桃的雌花柱头在V字期开始具有一定的可授性,八字期具有较强的可授性,是其授粉的最佳时期。
译  名：
Study on Pollen Viability and Stigma Receptivity of Two Walnut Cultivars Yangbidapao and Yunxingaoyuan
作  者：
HU Qing;XIAO Liang-jun;WU Tao;NING De-lu;CHEN Shao-yu;The Woody Oil Engineering Research Center of Yunnan Province;Yunnan Academy of Forestry;
关键词：
Juglans sigillata;;Yangbidapao;;Yunxingaoyuan;;Pollen viability;;Stigma receptivity
摘  要：
【Objective】The pollen viability and stigma receptivity of two walnut cultivars( J. sigillata cv. ‘Yangbidapao'and J. sigillata ×regia cv. ‘Yunxingaoyuan') planted in Kunming were studied in this paper. 【Method】The period and conditions( room temperature,coldstorage and frozen-storage) for keeping high pollen viability were detected by triphenyltetrazolium chloride( TTC) staining method. The stigma receptivity and optimal pollination period were evaluated by benzidine-H2O2,peroxidase-esterase reagent and sudan Ⅲ staining method.【Result】The results showed that the pollen viability of Yunxingaoyuan was higher than that of Yangbidapao at the same storage condition.The cold-storage was conducive to keeping high pollen viability,and which of Yangbidapao and Yunxingaoyuan were decreased to 10. 0 %and 18. 3 % respectively after seven days. 【Conclusion】The peroxidase-esterase reagent method was the best way to monitor the stigma receptivity,and the best period of pollination was the stigma opening angle ranging from 45 to 120 degrees.

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