中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 鳜亲环蛋白A在传染性脾肾坏死病毒增殖中的作用 水产学报 2016,40 (1) 100-109
Position: Home > Articles > Effect of mandarin fish cyclophilin A on the multiplication of infectious spleen and kidney necrosis virus Journal of Fisheries of China 2016,40 (1) 100-109

鳜亲环蛋白A在传染性脾肾坏死病毒增殖中的作用

作  者：
胡先勤;付小哲;董星星;涂加钢;赵丽娟;林强;李宁求;林蠡
单  位：
华中农业大学水产学院;中国水产科学研究院珠江水产研究所
关键词：
鳜;亲环蛋白A;传染性脾肾坏死病毒;环孢素A;基因克隆;病毒增殖
摘  要：
为研究鳜亲环蛋白(CypA)在传染性脾肾坏死病毒(ISKNV)感染中的作用,通过RT-PCR克隆了CypA全长开放读码框(SC-CypA)。结果表明SC-CypA基因全长495 bp,编码164个氨基酸,分子量为17.59 ku。通过Blast比对和同源性进化分析,发现其与人、鼠、原鸡和斑点鲖等物种的CypA具有高度相似性,表明SC-CypA属于CypA家族的成员。环孢素A(CsA)具有免疫抑制作用,是CypA的抑制剂。结果发现,CsA浓度与ISKNV增殖具有一定的剂量依赖关系;CsA作用不同时间对ISKNV均有抑制效果;CsA对ISKNV感染诱导的细胞因子的表达具有调节作用,能抑制IL-1β、IL-8、IL-18和ISG15的表达。研究表明,宿主的CypA对ISKNV的增殖起着重要的作用,通过添加其抑制剂CsA能有效抑制病毒的增殖。
译  名：
Effect of mandarin fish cyclophilin A on the multiplication of infectious spleen and kidney necrosis virus
作  者：
HU Xianqin;FU Xiaozhe;DONG Xingxing;TU Jiagang;ZHAO Lijuan;LIN Qiang;LI Ningqiu;LIN Li;College of Fisheries,Huazhong Agricultural University,Freshwater Aquaculture Collaborative Innovation Center of Hubei Province;School of Animal Sciences and Nutritional Engineering,Wuhan Polytechnic University;Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Fishery Drug Development,Ministry of Agriculture,Key Laboratory of Aquatic Animal Immune Technology,Guangdong Province;
关键词：
Siniperca chuatsi;;cyclophilin A;;infectious spleen and kidney necrosis virus;;cyclosporin A;;molecular clone;;virus replication
摘  要：
It has been shown that host protein cyclophilin A(CypA) played a key role in the replication of several viruses. In order to address the function of Mandarin fish CypA(SC-CypA) in infectious spleen and kidney necrosis virus(ISKNV) replication,the full ORF of SC-Cyp A was first cloned and characterized. The ORF of SCCyp A encoded a polypeptide of 164 amino acids with calculated molecular weight of 17.59 ku. The deduced amino acid sequences of the SC-CypA shared highly conserved structures with Cyp As from other species,indicating that SC-Cyp A should be a new member of the CypA family. Cyclosporin A(CsA) is a specific inhibitor of CypA. ISKNV replication was inhibited by the addition of CsA in a manner of dose dependence. CsA could effectively inhibit the replication of ISKNV at different time points after ISKNV infection,and the strongest inhibitory effect was observed at 72 h poi. The addition of CsA could inhibit the mRNA expressions of IL-1β,IL-8,IL-18 and ISG15 in CPB cells after the infection of ISKNV. These suggest that SC-CypA plays a key role in the ISKNV replication,and CsA could effectively inhibit the replication of ISKNV.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46