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Position: Home > Articles > Prevalence of swine hepatitis E in the selected pig farms in Yunnan Province Animal Husbandry & Veterinary Medicine 2015,47 (10) 10-13

云南省部分猪场猪戊型肝炎的流行情况调查

作  者:
严红亚;张佳;高利波;尹革芬;舒相华;杨贵树;李文贵
单  位:
云南农业大学动物科学技术学院
关键词:
猪;戊型肝炎病毒;流行;同源性分析;云南
摘  要:
为了解云南省猪群戊型肝炎感染情况,从云南省8个猪场采集166份新鲜猪粪便样品,用套式RT-PCR技术对戊型肝炎病毒(HEV)核酸进行检测,结果 21份样品阳性,阳性率为12.7%,感染率较高。通过PCR产物片段的测序分析,去除重复序列后得到11株基因序列。同源性分析结果表明,这11株毒株序列与选定的Gen Bank中基因1型、2型、3型和4型参考序列的核苷酸序列同源性分别为85.6%~87.8%,85.6%~87.8%,85.6%~95.6%和92.8%~98.9%,说明这些株毒株属于基因4型,与中国其他地区分离的毒株同源性最高,证实HEV的流行具有地理分布的特征。
译  名:
Prevalence of swine hepatitis E in the selected pig farms in Yunnan Province
作  者:
YAN Hongya;ZHANG Jia;GAO Libo;YIN Gefen;SHU Xianghua;YANG Guishu;LI Wengui;College of Animal Science and Technology,Yunnan Agricultural University;
关键词:
pig;;Hepatitis E virus;;prevalence;;phylogenetic analysis
摘  要:
To determine Hepatitis E virus( HEV) infection in pig farms of Yunnan Province,166 swine feces samples were randomly collected from eight pig farms and detected by nested RT- PCR. The results showed that 21 samples were positive and the total positive rate was12. 7%. Based on sequence analysis,we obtained 11 different sequences. The homology analysis indicated that 11 strains share 85. 6% ~87. 8%,85. 6% ~ 87. 8%,85. 6% ~ 95. 6% and 92. 8% ~ 98. 9% nucleotide homology with the selected sequences of genotype 1,2,3and 4,respectively,suggesting that all these isolated HEV belong to genotype 4 and share the highest homology with strains isolated in other parts of China. The result revealed that the prevalence of HEV has a characteristic geographic distribution.

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