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Chinese Journal of Preventive Veterinary Medicine
1998
(3)
肾型鸡传染性支气管炎病毒免疫机制探讨
作 者：
江国托;康丽娟;王永坤;李学贞;李俭钊;田华;计慧萍;孙运本
单 位：
扬州大学农学院动物医学系;扬州大学农学院动物医学系,黑龙江省青年农场,大庆市禽蛋公司,大连种鸡场
关键词：
肾型鸡传染性支气管炎,免疫球蛋白、抗体形成曲线
摘 要：
用自华东地区分离并鉴定的一株与标准肾型鸡传染性支气管炎病毒Ｎ１１５血清型相近的流行株Ｃ９００１试验。以饱和硫酸铵盐析－Ｓｅｐｈａｒｏｓｅ４Ｂ－ＤＥＡＥ纤维素－ＳｅｐｈａｄｅｘＧ１００等处理后获取高度纯化的鸡抗肾型鸡传染性支气管炎病毒免疫球蛋白；用肾型鸡传染性支气管炎病毒灭活疫苗免疫易感鸡，检测不同免疫时期的抗体并绘制抗体形成曲线，同时用Ｃ９００１强毒感染易感鸡，检测攻毒后不同时间的病毒抗体，绘制抗体形成曲线；用Ｃ９００１强毒对易感鸡人工致病，采用免疫荧光试验及免疫酶组化染色比较１１种不同的脏器中，在不同发病阶段的病毒复制与分布动态。分别从鸡抗肾型鸡传染性支气管炎病毒主要免疫球蛋白成分、肾型鸡传染性支气管炎病毒疫苗免疫及强毒感染后的抗体形成曲线及肾型鸡传染性支气管炎病毒在鸡体内不同器官、不同病程中的复制与分布规律等几个方面对肾型鸡传染性支气管炎病毒的免疫机制作了初步探讨。结果表明鸡抗肾型鸡传染性支气管炎病毒免疫球蛋白成分与抗呼吸道型鸡传染性支气管炎病毒不同，主要为ＩｇＧ；肾型鸡传染性支气管炎病毒疫苗免疫后第５天可检测到血清抗体，抗体水平高峰期出现在免疫后第１０～２０天，免疫保护力至少可维持１个月以上，强毒感染?
译 名：
Immunological Mechanism of Nephrosis Infectious Bronchitis Virus
作 者：
JIANG Guotuo,KANG Lijuan,WANG Yongkun et al (Department of Animal Sciences,Agriculture College of Yangzhou University,Yangzhou 225009)
关键词：
Nephrosis infectious bronchitis Immunogloulin Antibody formation curves
摘 要：
Once local strain of Nephrosis Infectious Bronchitis Virus (NIBV)designated as C 9001 was used in this study.Immunogloblin against NIBV was salted out and purified by Saturated Ammonium Sulfate and Sepharose 4B,DEAE Cellulose and Sephadex G100.Antibody fomation curves were pictured by the detection of serum antibodies after the vaccing of NIBV vaccine and inoculation of virus.The NIBV in eleven kinds of NIB pathologic organs of different disease period were detected using Immunoezyme Histochemistry and Immunofluorescent tests. Results demonstrated that the major antibody against NIBV was Immunoglobulin G.the titer limit of antibody in chickens inoculated with NIBV was 2 -4 to 2 -7 in Indirect Hemagglutimotion detection and the highest titer of antibody in chickens immunized with NIBV vaccine was detected at 10 to 20 days after vaccing.The order of the titer of NIBV in eleven kinds organs was:Kindy,Tracheal,Lung,Cloaca,Testicle,Liver,Thymus,Bursa,Oviduct,Spleen,and Heart,respevtivly.
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