单 位：
天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室;天津商业大学生物技术与食品科学学院
关键词：
三叶委陵菜;液相色谱-质谱/质谱联用;黄酮;橙皮苷;金丝桃苷
摘 要：
利用75%乙醇作为溶剂,采用微波辅助法提取三叶委陵菜黄酮,黄酮样品经D101树脂纯化,采用高效液相色谱分离、串联四极杆质谱负离子方式检测分析。根据相对分子质量、二级质谱碎片、液相色谱保留时间等信息,从三叶委陵菜中测定出了二苯乙烯苷、槲皮素-3,7-芸香糖鼠李糖苷、牡荆素、芦丁、金丝桃苷、橙皮苷、槲皮苷和山奈酚-3-芸香糖苷8种黄酮类成分。
译 名：
Determination of Flavonoids from Potentilla freyninan by HPLC-MS/MS
作 者：
LI Rong,ZHOU Yuan,YANG Li-chen,JIANG Zi-tao*(Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China)
关键词：
Potentilla freyninan;HPLC-MS/MS;flavonoids;hesperidin;hyperoside
摘 要：
Total flavonoids were obtained from Potentilla freyninan by microwave-assisted extraction method using 75% ethanol as the extraction solvent and purified with D101 macroporous resin.The purified total flavonoids were analyzed by high performance liquid chromatography tandem quadrupole mass spectrometry(HPLC-MS/MS) in the negative ion monitoring mode.Based on relative molecular weight,MS2fragmentation pattern,and retention time in liquid chromatography,eight flavonoids in P.freyninan were identified,including 2,3,5,4’-tetrahydroxystilbane-2-O-β-D-glucoside,quercetin-3,7rutinosogalactoside,apigenin-8-C-glucoside,rutin,hyperoside,hesperidin,quercitrin,and kaempferol-3-rutinoside.
