当前位置: 首页 > 文章 > 羟基喜树碱对人白血病K562细胞增殖和凋亡的影响 安徽农业科学 2010,38 (9) 4546-4549
Position: Home > Articles > Effects of Campotothecin(CPT) on Multiplication and Apoptosis of Human Leukemic K562 Cells Journal of Anhui Agricultural Sciences 2010,38 (9) 4546-4549

羟基喜树碱对人白血病K562细胞增殖和凋亡的影响

作  者:
邵淑丽;吴敏;武广慧
单  位:
齐齐哈尔大学生命科学与工程学院
关键词:
白血病;K562细胞;羟基喜树碱;细胞增殖;细胞凋亡
摘  要:
[目的]研究羟基喜树碱对人白血病K562细胞增殖与凋亡的影响。[方法]通过光学显微镜、荧光显微镜、电子显微镜、琼脂糖凝胶电泳、流式细胞术等方法,探讨不同浓度羟基喜树碱作用不同时间后,K562细胞凋亡的情况。[结果]羟基喜树碱能够诱导K562细胞凋亡,作用48h的最佳浓度为8.0μg/ml。羟基喜树碱可影响K562细胞增殖周期中的S期,即DNA合成期,细胞在此期停滞,诱导其发生凋亡。[结论]为将羟基喜树碱应用于临床治疗白血病提供前期试验依据。
译  名:
Effects of Campotothecin(CPT) on Multiplication and Apoptosis of Human Leukemic K562 Cells
作  者:
SHAO Shu-li et al (College of Life Science and Engineering,Qiqihar University,Qiqihar,Heilongjiang 161006)
关键词:
Leukemic; K562 cell; Campotothecin(CPT); Multiplication; Apoptosis
摘  要:
[Objective]The research aimed to study the effects of campotothecin on multiplication and apoptosis of human leukemic K562 cells. [Method] The multiplication and apoptosis status of K562 cells treated different time by different concentration campotothecin were observed by light microscope,fluorescence microscope,TEM,agrose gel electrophoresis of DNA and flow cytometry. [Result]The results showed that campotothecin could induce K562 cellls apoptosis,and the best concentration was 8.0 μg/ml for 48 h. Campotothecin could affect the S phase among cellular multiplication,cell could be blocked by campotothecin and then apoptosis in this phase. [Conclusion] The study can provide previous experiment basis for campotothecin applying clinical treatments.

相似文章

计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊