Position: Home > Articles > Extraction of Flavonoids from Herba houttuyniae Using Ethanol-Ammonium Sulfate Aqueous Two Phase System
Hubei Agricultural Sciences
2017
(24)
4844-4846
乙醇-硫酸铵双水相提取鱼腥草总黄酮
作 者：
郝红英;周芳;王改利;高阳逸;丁小娟
单 位：
黄河科技学院医学院
关键词：
鱼腥草(Herba houttuyniae);双水相;总黄酮;提取工艺
摘 要：
通过单因素试验优化了影响乙醇-硫酸铵双水相提取鱼腥草(Herba houttuyniae)总黄酮提取率的因素,进一步通过正交试验确定了各因素的影响程度,顺序为乙醇浓度>提取温度>提取时间>料液比。优化后的提取条件为脱脂鱼腥草粉末2.0 g,乙醇用量32.50%(w)、硫酸铵16.00%(w)、料液比1∶15、提取温度60℃、提取时间30 min,在该条件下,鱼腥草总黄酮提取率为3.16%。
译 名：
Extraction of Flavonoids from Herba houttuyniae Using Ethanol-Ammonium Sulfate Aqueous Two Phase System
作 者：
HAO Hong-ying;ZHOU Fang;WANG Gai-li;GAO Yang-yi;DING Xiao-juan;Medical School,Huanghe Science and Technology College;
关键词：
Herba houttuyniae;;aqueous two phase system;;total flavonoids;;extraction technology
摘 要：
The factors affecting the extraction yield of flavonoids were optimized by single factor test and orthogonal design to investigate the effect on the extraction technology of flavonoids from herba houttu-yniae using ethanol-ammonium sulfate aqueous two phase system. The influence degree of each factor was determined by orthogonal experiment :ethanol content>extraction temperature >extraction time >solid-liquid ratio. The optimal extraction technology was that skim herba houttuyniae was 2.0 g,ethanol 32.50%(w),ammonium sulfate 16.00%(w),solid-liquid ratio 1∶15,temperature 60 ℃,and time was 30 min. Under this condition, the extraction rate of flavonoids was 3.16%.
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