单 位：
西南林业大学西南地区生物多样性保育国家林业局重点实验室
摘 要：
为揭示植物逆境保护酶系统变化与生根的关系,取吉贝2 a生采穗圃中当年生枝条为插穗,比较3种配方处理插穗生根质量及不同生根阶段中保护酶系统超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性变化规律。结果显示:(1)3种配方处理插穗后,愈伤组织出现时间、第1条根出现时间、生根高峰期较对照分别提早0.80 d、1.30 d及2.60 d以上:(2)较好促根配方对吉贝插穗生根效果也不同,1#处理生根系数高于其它处理,方差分析差异达显著;(3)SOD、POD在生根过程中均呈现"升高一下降"趋势,且均在根源基诱导期达到峰值,活性值与对照差异显著,生根进程中酶活值达极显著;(4)CAT活性呈"升高—下降—升高"趋势,峰值同样出现在根源基诱导期,酶活值与扦插起始期或CK差异显著;(5)逆境下自由基通过SOD活性增加歧化后,H_2O_2产物由POD、CAT增活共同清除,从而降低氧化胁迫。研究表明,1#配方生根质量高于其它配方,且配方处理能够促进逆境下保护酶活性的升高,有效清除自由基积累,并维持一定的动态平衡促进生根。
译 名：
Effects of Three Rooting Formula on Rooting Rate and Protective Enzyme Activities in Ceiba Pentandra Cutting
作 者：
GAO Zhu~(1,2),YAN Yi~1,WU Jian-rong~1,MA Huan-cheng~(1*),ZHENG Yuan~1 (1.Key Laboratory of Biodiversity Conservation in Southwest China of State Forestry Administration, Southwest Forestry University,Kunming 650224,China;2.Institute of Biological Resources,Jiangxi Academy of Sciences,Nanchang 330029,China)
关键词：
Ceiba pentandra;;formula treatment;;rooting of cutting;;protective enzyme system
摘 要：
To reveal the protective enzyme system change and the relationship between plant roots,annual stiff branches in 2-year-old cutting orchard of Ceiba pentandra were taken as materials in this experiment.The effects of three formulations on dealing with root cuttings in terms of root quality and the changes of SOD, POD and CAT activities in different were compared.The result showed:(1) The callus emergence time,first bar emergence time,rooting peak were 0.80 d,1.30 d and 2.60 d earlier than those of CK.(2) Better root formula had different effects on rooting of Ceiba pentandra.The 1# treatment had higher root coefficient than other treatments,and the difference was significant.(3) During the rooting process,SOD,POD activity showed a tendency of "higher-lower".The base peak appeared in the root primordial induction period,and the activity values were of significant difference.It was of extremely significant difference during the rooting process.(4) CAT activity showed a tendency of "higher-down -increasing" during the rooting process.The base peak also appeared in the root primordial induction period,whose activity value was significantly different from that in the starting cutting period or that of CK.(5) SOD activity under stress,by increasing free radical,was disproportionaed.POD,CAT activities increased and cleared product of H_2O_2,thereby reduced oxidative stress.
