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Position: Home > Articles > Observation of the structure of Cysticercus cellulosae from the experimentally infected swine Chinese Veterinary Science 2008,38 (7) 605-611

人工感染猪囊尾蚴的组织结构观察

作  者:
孙晓林;骆学农;景志忠;王佩雅;才学鹏
单  位:
中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室
关键词:
猪囊尾蚴;透射电镜;石蜡切片;HE染色
摘  要:
为了解成熟猪囊尾蚴的组织结构,进一步研究其特异性抗原定位,对猪带绦虫病患者用槟榔、南瓜子驱虫,采集虫卵,人工感染猪,收集新鲜猪囊尾蚴,石蜡切片,HE染色后进行了光镜观察,并用胆汁法翻出头节,超薄切片,进行了透射电镜观察。光镜观察表明,囊尾蚴有2个囊腔,较小的囊腔包括头节和颈节,较大的囊腔包括囊壁和囊液,囊壁外层具有绒毛,并有宿主结缔组织的成分;电镜观察表明,翻出头节的囊尾蚴除与已报道的未翻出头节的囊尾蚴结构相同外,最突出的特点是神经索和排泄管并行,石灰小体呈板层状。
译  名:
Observation of the structure of Cysticercus cellulosae from the experimentally infected swine
作  者:
SUN Xiao-lin1,2,LUO Xue-nong1,JING Zhi-zhong1,WANG Pei-ya1,CAI Xue-peng1(1.Key Laboratory of Veterinary Parasitology of Gansu Province / State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;2.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China)
关键词:
Cysticercus cellulosae;transmission electron microscope;paraffin section;HE staining
摘  要:
To understand the structure of mature Cysticercus cellulosae so as to further study the specific antigen localization in C.cellulosae,the patients with taeniasis suis were treated with the decoction of areca and pumpkin seeds to collect the eggs of Taenia solium.The mature C.cellulosae were gathered from the pigs infected with the eggs.The structure of C.cellulosae was observed by light microscope with the paraffin section and the HE staining.Ultrathin section of evaginated scolex of the cysticercus was observed by transmission electron microscope(TEM).The cysticercus consisted of two chambers.The small one included scolex and collum segment,and the larger was conformed by cystic fluid and cystic wall.There were microtriche and connective tissue of the host on the outer layer of cystic wall.The TEM results indicated that the most prominent characteristics of the evaginated cysticercus were the parallel arrangement of nerve cord with excretory duct and the board-layered calcareous corpuscles.

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