Position: Home > Articles > Studies on the Recessive Genie Male Sterility (GMS) and Its Genetic Markers in Rapeseed (Brassica napus L. ) Ⅱ. DNA Molecular Confirmation for P6-9 Purple Stem Gene Linked to Male Fertility Gene of GMS
Acta Agronomica Sinica
1999,25
(6)
669-673
甘蓝型油菜隐性核不育遗传标记的初步研究 Ⅱ.P6-9紫茎基因与可育基因连锁的分子证据
作 者：
涂金星;傅廷栋;郑用琏;杨光圣;马朝芝;杨小牛
单 位：
华中农业大学作物遗传改良国家重点实验室
关键词：
油菜核不育;紫茎;RAPD标记
摘 要：
为了进一步证实P6-9紫茎性状与可育性状的连锁关系,本试验选用S45A×自1968的BC_1F_1群体,利用BSA分析法,筛选到两个与可育基因Ms_1连锁的RAPD片段:UBC_(158.580)和UBC_(187.880).通过对可育株群体的分析,证明了P6-9紫茎基因Pur与Ms_1基因以及UBC_(158.580)和UBC_(187.880)位于同一连锁群上;对不育株分析得出其在同一连锁群上的精确位置,Pur(3.25cM)—Ms_1(8.67cM)—UBC_(158.580)(8.78.M)—UBL_(18,880).
译 名：
Studies on the Recessive Genie Male Sterility (GMS) and Its Genetic Markers in Rapeseed (Brassica napus L. ) Ⅱ. DNA Molecular Confirmation for P6-9 Purple Stem Gene Linked to Male Fertility Gene of GMS
作 者：
Tu Jinxing Fu Tingdong Zheng Yonglian Yang Guangsheng Ma Chaozhi Yang Xiaoniu(State Key Lab for Genetic Improvement of Crops Huazhang Agricultural University, Hubei Wuhan, 430070)
关键词：
GMS; Purple stem; RAPD marker
摘 要：
In order to identify the linkage relationship between P6-9 purple stem gene and the male fertility gene, the BC1F1 population from S45AxxxxxxxxxxxZi 1968 was investigated by BSA (Bulked Segregant Analysis) and found out two RAPD fragments, UBC158.580 and UBC187.880, linked to the purple stem gene and the male fertile gene(Ms1). The results show that these four genes are located the same linkage group, and the order is as follow: Pur(3. 25 cm)-Ms1(8. 67 cm)-UBC158.580(8.78cm)-UBC187.880.
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