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Position: Home > Articles > Isolation of an Endophyte Bacillus megaterium from Cowpea Flowers Journal of Anhui Agricultural Sciences 2015,43 (32) 23-24

一株豇豆花内生巨大芽孢杆菌的分离鉴定

作  者:
熊燕妮;江瑞晶;方浩;韩雪;王丹
单  位:
江汉大学生命科学学院
关键词:
豇豆;内生菌;巨大芽孢杆菌;染色;序列分析
摘  要:
从武汉豇豆种植区的豇豆花中筛选出花内生菌(命名为whcfs1)。它在豇豆花各花期花中为优势内生菌。营养体可以在23~49℃温度生长;经染色鉴定,它为革兰氏阳性芽孢杆菌;在16S r DNA扩增产物测序后,经软件分析为巨大芽孢杆菌,生化试验显示该菌生化特征符合巨大芽孢杆菌特征。该试验分离得到的内生巨大芽孢杆菌(whcfs1)有望成为豇豆基因工程益生菌及生防菌的理想宿主菌株。
译  名:
Isolation of an Endophyte Bacillus megaterium from Cowpea Flowers
作  者:
XIONG Yan-ni;JIANG Rui-jing;FANG Hao;HAN Xue;College of Life Science,Jianghan University;
关键词:
Cowpea;;Endophytic bacteria;;Bacillus megaterium;;Staining;;Sequence analysis
摘  要:
The endophyte bacteria( whcfs1) was isolated from the cowpea flowers in Wuhan,which was the dominant strain in the cowpea florescence. The proper temperature range for the endophyte bacteria culture was 23- 49 ℃ and the gram staining and spore staining were positive.The sequence of 16 S r DNA was sequenced and the results showed the endophyte bacteria was belong to Bacillus megaterium and the biochemical character was agree to the Bacillus megaterium. The endophyte bacillus megaterium( whcfs1) in this paper was an ideal candidate of the host for cowpea engineered probiotics and biocontrol bacteria.

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