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当前位置: 首页 > 文章 > 毛白杨PtoMnSOD基因克隆及生物信息学分析 分子植物育种 2022,20 (1) 105-112
Position: Home > Articles > Cloning and Bioinformatics Analysis of PtoMnSOD Gene in Populus tomentosa Molecular Plant Breeding 2022,20 (1) 105-112

毛白杨PtoMnSOD基因克隆及生物信息学分析

作  者：
韩佩尧;田彦挺;赵烨;纪清巨;李娟;郭琪;侯荣轩;孙宇涵;李云
单  位：
北京林业大学生物科学与技术学院林木分子设计育种高精尖创新中心林木育种国家工程实验室林木花卉遗传育种教育部重点实验室;盐山县自然资源和规划局;沧州市自然资源和规划局种苗站
关键词：
毛白杨;MnSOD基因;生物信息学分析
摘  要：
为了揭示毛白杨锰超氧化物歧化酶基因PtoMnSOD在植物抗逆中的功能。本研究通过RT-PCR方法获取毛白杨PtoMnSOD基因序列,并对该基因及其编码蛋白进行生物信息学分析。结果显示:PtoMnSOD基因开放阅读框(ORF)为699 bp,编码232个氨基酸,具有PF00081和PF02777两个保守结构域,属于MnSOD亚家族。其编码的蛋白含有20个磷酸化位点,是无跨膜结构、无信号肽且定位于线粒体的不稳定亲水性蛋白。同源序列比对显示,不同物种的MnSOD氨基酸序列具有很强的保守性。研究结果为进一步开展毛白杨锰超氧化物歧化酶基因PtoMnSOD的功能研究奠定了理论基础。
译  名：
Cloning and Bioinformatics Analysis of PtoMnSOD Gene in Populus tomentosa
作  者：
Han Peiyao;Tian Yanting;Zhao Ye;Ji Qingju;Li Juan;Guo Qi;Hou Rongxuan;Sun Yuhan;Li Yun;Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, National Engineering Laboratory for Tree Breeding, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, Beijing Forestry University;Seedling Station of Cangzhou Bureau of Natural Resources and Planning;Natural Resources and Planning Bureau of Yanshan County;
关键词：
Populus tomentosa;;MnSOD gene;;Bioinformatics analysis
摘  要：
In order to reveal the function of manganese superoxide dismutase gene PtoMnSOD in plant stress resistance. In this study, the PtoMnSOD gene sequence of Populus tomentosa was obtained by RT-PCR, and the bioinformatics analysis of the gene and its encoded protein was carried out. The results showed that the open reading frame(ORF) of PtoMnSOD gene was 699 bp, encoding 232 amino acids, with two conserved domains, PF0-0081 and PF02777, belonging to MnSOD subfamily. The protein encoded contains 20 phosphorylation sites. It was an unstable hydrophilic protein without transmembrane structure and signal peptide and located in mitochondria.Homologous sequence alignment showed that the amino acid sequences of MnSOD from different species were highly conserved. The results lay a theoretical foundation for further study on the function of manganese superoxide dismutase gene PtoMnSOD in Populus tomentosa.

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