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Position: Home > Articles > The research methods in soil microbial molecular ecology Soil and Fertilizer Sciences in China 2008,2008 (6) 1-4+14

土壤微生物分子生态学研究方法

作  者:
李世贵;吕天晓;顾金刚;姜瑞波;牛永春
单  位:
中国农业科学院农业资源与农业区划研究所;东北农业大学资源与环境学院
关键词:
土壤微生物生态学;微生物分子生态学;核酸探针杂交;梯度凝胶电泳;PCR
摘  要:
主要介绍了目前在土壤微生物生态学研究中的常用分子生物学方法,包括核酸探针杂交技术、基于PCR技术的研究方法、特异DNA片段的序列分析、DNA扩增片段梯度凝胶电泳检测技术等。
译  名:
The research methods in soil microbial molecular ecology
作  者:
LI Shi-gui1,Lü Tian-xiao2,GU Jin-gang1,JIANG Rui-bo1,NIU Yong-chun1(1.Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081;2.Resources and Environmental Sciences Courage,Northeast Agricultural University,Harbin 150030)
关键词:
soil microbial ecology;microbial molecular ecology;nucleotide probe hybridization;gradient gel electrophoresis;PCR
摘  要:
This article mainly introduced some molecular biology methods that were commonly used in the soil microbial ecology research at present. These methods included that the nucleic acid probe hybrid technology,PCR-based research technique,the special DNA fragment sequence analysis,DNA amplified fragment gradient electrophoresis examination technology and so on.

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