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Position: Home > Articles > Regeneration of Transgenic Indica Rice Plants with a Cecropin B Gene and a bar Gene Chinese Journal of Rice Science 2000,14 (3)

转抗菌肽B基因和bar基因籼稻植株的再生

作  者:
王慧中;华志华;高振宇;颜美仙;钱前;黄大年
单  位:
杭州师范学院生物系!浙江杭州310012中国水稻研究所,浙江杭州310006,中国水稻研究所!浙江杭州310006,中国水稻研究所!浙江杭州310006,中国水稻研究所!浙江杭州310006,中国水稻研究所!浙江杭州310006,中国水稻研究所!浙江杭州310006;杭州师范学院生物系
关键词:
水稻;bar基因;抗菌肽B基因;转基因植株;抗病性;白叶枯病
摘  要:
运用基因枪法将含有 bar基因和 cecropin B基因的质粒 p CB1导入籼稻品种特青的幼胚细胞 ,筛选后得到 3株转基因植株。PCR检测和 Southern杂交结果表明 ,外源基因已整合到水稻基因组中。转化当代植株表现出对 Basta很强的抗性 ,同时也增强了对水稻白叶枯病的抗性
译  名:
Regeneration of Transgenic Indica Rice Plants with a Cecropin B Gene and a bar Gene
作  者:
WANG Hui zhong 1,2 , HUA Zhi hua 2, GAO Zhen yu 2, YAN Mei xian 2, QIAN Qian 2, HUANG Da nian 2 ( 1Department of Biology, Hangzhou Normal College,Hangzhou 310012, China; 2China National Rice Research Institute,Hangzhou 310006, China)$$$
关键词:
rice; bargene; cecropin B gene; transgenic plant; disease resistance; Xanthomonas campestris pv.oryzae
摘  要:
Immature embryos of indica rice variety Teqing were bombarded with the plasmid pCB1 containing a bar gene and a cecropin B gene. Three transgenic plants were regenerated after selection. The results of PCR assay and Southern blot analysis showed that foreign genes had been integrated into the rice genome. Transformed plants showed high resistance to herbicide Basta and Xanthomonas campestris pv. oryzae.

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