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Position: Home > Articles > Phylogenetic Relationship of Bos grunniens in Qinghai Based on Mitochondrial cox1 and nad1 Genes China Animal Husbandry & Veterinary Medicine 2013,40 (2) 151-154

基于线粒体cox1和nad1基因对青海牦牛种系发育关系的研究

作  者:
钱建新;钱和平;徐志伟;袁彬彬
单  位:
湖南农业大学动物科技学院;湖南省湘乡市畜牧水产局;湖南省南县畜牧水产局
关键词:
青海牦牛;线粒体DNA;cox1基因;nad1基因;种系发育关系
摘  要:
本研究选取4头青海牦牛作为研究对象,用2对引物扩增青海牦牛的线粒体细胞色素c氧化酶第1亚基(cox1)基因部分序列(pcox1)和烟酰胺腺嘌呤二核苷酸(NADH)脱氢酶亚单位1基因(nad1)部分序列(pnad1),并用pcox1和pnad1序列重构青海牦牛与其他牛的进化关系。对测定获得序列应用ClustalX 1.81程序进行比对,然后用Phylip 3.67程序MP法,并用Puzzle 5.2程序构建最大似然树。结果表明,所获得的4头牦牛样品pcox1和pnad1基因序列分别为823和837bp,种系发育分析表明,青海牦牛样品与GenBank已知的牦牛位于同一分支,与其他牛所属分支相隔较远。
译  名:
Phylogenetic Relationship of Bos grunniens in Qinghai Based on Mitochondrial cox1 and nad1 Genes
作  者:
QIAN Jian-xin1,QIAN He-ping1,XU Zhi-wei2,YUAN Bin-bin3(1.Animal Husbandry and Aquatic Products Bureau of Xiangxiang,Xiangxiang 411400,China; 2.Animal Husbandry and Aquatic Products Bureau of Nanxian,Yiyang 413200,China; 3.College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China)
关键词:
B.grunniens;mitochondrial DNA;cox1 gene;nad1 gene;phylogenetic relationship
摘  要:
The objectives of the present study were to amplify and analyse the mitochondrial cytochrome c oxidase subunit 1(cox1) and NADH dehydrogenase subunit 1(nad1) genes among Bos grunniens(B.grunniens)isolates from Qinghai province in China,and to study its phylogenetic relationships with Bos of the other species using cox1 and nad1 genesss sequences.The partial cox1(pcox1) and nad1(pnad1) genes were amplified from each B.grunniens sample,and pcox1 and pnad1 gene sequences were aligned using the ClustalX 1.81.Maximum parsimony(MP),maximum likelihood(ML) trees were constructed using the software Phylip 3.67 version 4.0 and ML tree was also constructed using Puzzle 5.2.The lengths of all the pcox1 and pnad1 gene sequences were 823 and 837 bp,respectively.Phylogenetic analyses showed that the B.grunniens samples from Qinghai were clustered in the same clade.The results of this study provided foundation for further studies on molecular genetics and variation of B.grunniens.

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