中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 混合反胶团萃取α-淀粉酶 食品科学 2011 (21) 214-217
Position: Home > Articles > Purification of α-Amylase Using CTAB/Tween-60 Mixed Reverse Micellar System FOOD SCIENCE 2011 (21) 214-217

混合反胶团萃取α-淀粉酶

作  者：
高树刚;宋伟明;安红
单  位：
齐齐哈尔大学化学化工学院;淮海工学院化工学院
关键词：
十六烷基三甲基溴化铵(CTAB);吐温-60;反胶团;α-淀粉酶;萃取
摘  要：
以十六烷基三甲基溴化铵(CTAB)和脱水山梨醇单硬脂酸酯聚氧乙烯醚(Tween-60)为混合表面活性剂溶于正丁醇-异辛烷中构成反胶团系统,萃取纯化α-淀粉酶。研究不同萃取条件下,α-淀粉酶的萃取率。其中反胶团相组成为:ρ(CTAB+Tween-60)=4g/L;n(CTAB):n(Tween-60)=2.0:1.0;V(正丁醇):V(异辛烷)=1.0:1.0。水相组成为:α-淀粉酶配制的粗酶液,此时c(NaCl)=0.04mol/L,水相pH11.04。结果表明:萃取温度40℃、V(有机相):V(水相)=2.0:1.0、振荡时间10min时,α-淀粉酶萃取率可达91%;反萃取水相组成为c(NaCl)=2.5mol/L、pH4.5、V(水相):V(有机相)=1.0:2.0,反萃取振荡时间10min、温度50℃时,α-淀粉酶反萃取率可达65%。反胶团相可重复使用,当V(水相):V(有机相)=1.0:2.75时,反胶团第2次α-淀粉酶萃取率达到71%。
译  名：
Purification of α-Amylase Using CTAB/Tween-60 Mixed Reverse Micellar System
作  者：
GAO Shu-gang1,SONG Wei-ming2,AN Hong1 (1.Department of Chemical Engineering,Huaihai Institute of Technology,Lianyungang 222005,China; 2.College of Chemistry and Chemical Engineering,Qiqihar University,Qiqihar 161006,China)
关键词：
cetyltrimethylammonium bromide(CTAB);Tween-60;reverse micelle;α-amylase;extraction
摘  要：
Reverse micellar system consisting of the surfactants cetyltrimethyl ammonium bromide(CTAB) and Tween-60 in dissolved in n-butanol-isooctane mixture was used for α-amylase purification by reversed micellar extraction.Extraction efficiency of α-amylase was investigated under different extraction conditions using a reverse micellar phase consisting of 4 g/L(CTAB +Tween-60,2.0:1.0,n/n) in n-butanol-isooctane mixture(1.0:1.0,V/V) and an aqueous phase made up of crude α-amylase solution containing 0.04 mol/L NaCl.The extraction efficiency of α-amylase was 91% when the extraction temperature,ratio of organic phase to aqueous phase and oscillation time were 40 ℃,2.0:1.0 and 10 min,respectively.The reverse extraction efficiency of α-amylase was 65% after 10 min oscillation at 50 ℃ when the aqueous phase was comprised of aqueous phase and organic phase at a ratio of 1.0:2.0(V/V) at pH 4.5 containing 2.5 mol/L NaCl.The reverse micelles could be repeatedly used and the extraction efficiency of α-amylase at an aqueous phase-to-organic phase ratio of 1.0:2.75 remained 71% in the second use.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46