Position: Home > Articles > Antimicrobial activity of Serratia grimesii CNY-04 against Botrytis cinerea and characteristic analysis of antagonistic substances
Plant Protection
2013,39
(3)
73-77
格氏沙雷菌CNY-04对灰霉病菌作用机制及抑菌物质理化性质研究
作 者：
陆继臣;迟乃玉;张庆芳
单 位：
大连大学辽宁省海洋微生物工程技术研究中心;大连工业大学生物工程学院
关键词：
格氏沙雷菌;灰霉病菌;抑菌作用;理化性质
摘 要：
从蔬菜根际土壤中筛选到一株生防格氏沙雷菌(Serratia grimesii)CNY-04,利用抑菌圈法测定其对灰霉病菌(Botrytis cinerea Pers.ex Fr.)的抑制效果,通过抑制孢子萌发、影响菌丝正常生长试验对CNY-04的作用机制进行研究,并对CNY-04菌株相关性状及抑菌物质的理化性质进行了研究。结果表明,CNY-04菌株对灰霉病菌拮抗能力强且遗传稳定,抑菌圈直径达到34mm;CNY-04可对灰霉病菌菌丝结构造成破坏;可显著抑制灰霉病菌分生孢子萌发,抑制率为99%;CNY-04菌株不产生硝吡咯菌素、氢氰酸及水解酶类,可产生嗜铁素和蛋白酶;抑菌物质具有较好的热稳定性和酸碱稳定性。抑菌物质由灰霉病菌诱导产生,与已报道的沙雷菌属的作用机理完全不同。
译 名：
Antimicrobial activity of Serratia grimesii CNY-04 against Botrytis cinerea and characteristic analysis of antagonistic substances
作 者：
Lu Jichen1,2,Chi Naiyu2,Zhang Qingfang2(1.College of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China; 2.Dalian University,Liaoning Center of Marine Microbial Engineering and Technology,Dalian 116622,China)
关键词：
Serratia grimesii;Botrytis cinerea;antagonistic substance;physicochemical property
摘 要：
Serratia grimesii CNY-04 was screened from the rhizosphere soil of the vegetable.The antibacterial circle method was used for determination of the activity of CNY-04 against Botrytis cinerea.The study was carried out through the inhibition of spore germination and hyphal growth,and the related traits of S.grimesii CNY-04 were determined.The characteristic analysis of antagonistic substances was also studied.The results showed that the strain CNY-04 exhibited genetic stability and strong antagonistic ability.The antibacterial circle diameter was up to 34 mm.The mycelial structure of B.cinerea could be damaged by CNY-04.The strain CNY-04 significantly inhibited the spore germination of B.cinerea,with an inhibition rate of 99%.CNY-04 could not produce pyrrolnitrin,hydrocyanic acid,and hydrolases,but could produce siderophore and proteinase.The antibacterial material had a high thermal stability and acid-based stability.Antibacterial substances were induced by B.cinerea.The bacteriostatic mechanism was completely different from that previously reported.
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