作 者：
马兴乾;金鹏;冯新畅;李华涛;唐大木;刘云
单 位：
加拿大麦克马斯特大学医学院;东北农业大学动物医学学院
关键词：
光动力学疗法;血卟啉单甲醚;犬乳腺肿瘤细胞;凋亡
摘 要：
用HMME-PDT作用于犬乳腺肿瘤细胞株CHMm后,台盼蓝染色绘制各组细胞的生长曲线,放射免疫法检测PDT后不同时间点检测细胞IL-6、IL-2含量的变化。台盼蓝染色PDT组与对照组比较细胞死亡率明显上升,PDT后随时间延长IL-2含量增加,IL-6含量降低。说明PDT对犬乳腺肿瘤细胞株CHMm的杀伤效应可能是由于细胞IL-6、IL-2含量的变化而引起的。
译 名：
Studies on the effects of photodynamic therapy (PDT) on IL-6 and IL-2 in canine breast tumor cells
作 者：
MA Xing-qian1,JIN Peng1,FENG Xin-chang1,LI Hua-tao1,TANG Da-mu2,LIU Yun1(1.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China;2.Mcmaster University School of Medicine,Hamilton,Canada)
关键词：
photodynamic therapy;hematoporphyrin monomethyl ether;canine breast tumor cells;apoptosis
摘 要：
After canine breast tumor cell line CHMm was treated with HMME-PDT.Growth curve of cells in each group was drawn after trypan-blue staining.The changes in the levels of IL-6 and IL-2 were detected with radioimmunoassay method after PDT at different times.The cell death rate of PDT group with trypan-blue staining increased more obviously compared with controls.The levels of IL-2 increased but IL-6 decreased after PDT as time continued.The lethal effect of PDT on canine breast tumor cell line CHMm might be caused by the change in the levels of IL-6 and IL-2 in these cells.
