Position: Home > Articles > Isolation of Calpain3 and Characterization of Its Autolysis
FOOD SCIENCE
2006,27
(3)
65-70
Calpain3的初步提取和对其自动降解性的研究
作 者：
童敏;徐幸莲;周光宏
单 位：
南京农业大学农业部农畜产品加工与质量控制重点开放实验室
关键词：
钙激活酶3;自动降解;钙离子
摘 要：
骨骼肌特异性的Calpain3(又称Ncl-1、p94、CAPN3)是除μ-Calpain和m-Calpain以外,另一个可能与肉类嫩化有关的Calpains成员。由于Calpain3具有很强的自动降解性,使得对其的提取纯化和依钙性的研究很难进行。本实验从牛肉背最长肌中提取到了具有一定活性的Calpain3粗酶液,通过蛋白质免疫印迹法,对Calpain3的随时间的降解变化进行了研究,并对不同Ca2+浓度下Calpain3自动降解的变化进行了比较系统的探讨,研究发现Calpian3的自动降解分为四个连续性的过程:首先降解为89、68和58kDa的中间片断,然后进一步降解为55kDa的片断并在一定时间内保持不变,其降解的速度与Ca2+浓度和反应时间成正比。
译 名：
Isolation of Calpain3 and Characterization of Its Autolysis
作 者：
TONG Min,XU Xing-lian*,ZHOU Guang-hong(Key Laboratory of Agricultural and Animal Products Processing and Quality Control, Ministry of Agriculture,Nanjing Agricultural University, Nanjing 210095, China)
关键词：
calpain3;autolysis;calcium
摘 要：
Calpain 3 is a skeletal muscle-specific calpain in calpain family. Isolation of the intact 94kDa enzyme has been difficultto achieve due to its rapid autolysis, and uncertainty has arisen over its dependence for Ca2+ activity.In the study, calpain3 crudeextract was obtiained, and found that in the presence of Ca2+, calpain3 cleaved itself into several fragments. This indicated thatthe autolysis of calpain3 was dependent on the concentration of Ca2+. It was also found that the autolysis of calpain3 proceedsin four consecutives steps: 89, 68and 58kDa fragments are produced as intermediates before a stable 55kDa fragment appears.
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